| 不同活性氧产生途径对高糖诱导内皮细胞活性氧生成及高糖对细胞凋亡的影响 |
| 投稿时间：2014-06-24 修订日期：2014-07-09 点此下载全文 |
| 引用本文：王蕾,苏华斌,卢琼.不同活性氧产生途径对高糖诱导内皮细胞活性氧生成及高糖对细胞凋亡的影响[J].医学研究杂志,2015,44(2):81-84 |
| DOI：
10.3969/j.issn.1673-548X.2015.02.022 |
| 摘要点击次数: 1883 |
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| 基金项目:湖南省教育厅科研基金资助项目(13C739);湖南医药学院科研基金资助项目 |
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| 中文摘要:目的 探讨不同活性氧产生途径对高糖诱导的内皮细胞活性氧生成的影响以及高糖对内皮细胞凋亡的影响。方法 本实验分为正常对照组、高糖处理组、DPI处理组、别嘌呤醇处理组、鱼藤酮处理组和吲哚美辛处理组。用流式细胞仪检测各组细胞内活性氧的浓度;流式细胞仪及Hochest染色检测细胞凋亡;Western blot法检测NADPH氧化酶(nicotinamide adenine dinucleotide phosphate oxidase)亚单位NOX2、NOX4、p22phox、p47phox、p67phox及rac的蛋白表达。结果 与对照组相比, 高糖组、别嘌呤醇处理组、鱼藤酮处理组和吲哚美辛处理组内皮细胞活性氧的生成明显增高, 而DPI处理组内皮细胞活性氧的生成与对照组差异无统计学意义;与高糖组相比, DPI处理组内皮细胞活性氧的生成明显减少;而别嘌呤醇处理组、鱼藤酮处理组和吲哚美辛处理组与高糖组相比差异无统计学意义。与正常对照组相比, 高糖处理组细胞NOX4蛋白表达显著增高, 而NOX2、p47phox、p67phox、p22phox和Rac表达差异无统计学意义。与对照组相比, 高糖处理组细胞凋亡比例显著增高。结论 高糖可能通过增加活性氧的生成诱导内皮细胞的凋亡, 其中NADPH氧化酶可能为内皮细胞活性氧产生的主要来源。 |
| 中文关键词:高糖 活性氧 凋亡 NADPH氧化酶 黄嘌呤氧化酶 线粒体电子传递链 环氧合酶 |
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| Effect of Different Pathways of ROS on ROS and Apoptosis Induced by High Glucose in HUVECs |
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| Abstract:Objective To study the effect of different pathways of reactive oxygen species(ROS) on ROS induced by high glucose and to investigate effect of high glucose on apoptosis in HUVECs. Methods There were control group, high glucose group, DPI group, oxypurinol group, rotenone group and indometacin group in this study. Intracellular ROS level was detected by flow cytometry. Apoptosis was detected by flow cytometry and Hochest dyeing. The protein expression of NADPH oxidase (NOX2, NOX4, p22phox, p47phox, p67phox and rac) was detected by western blot. Resutls Compared with control group, intracellular ROS level of high glucose group, oxypurinol group, rotenone group and indometacin group were obviously increased, but there was no significant difference among DPI group and control group.Compared with control group, the protein expression of NOX4 was increased by high glucose, and there was no significant difference in the protein expression of NOX2, p22phox, p47phox, p67phox and rac. Compared with control group, apoptosis was increased by high glucose. Conclusion High glucose increases apoptosis possibly by increasing intracellular ROS level, and NADPH oxidase may be the main resource of ROS. |
| keywords:High glucose ROS Apoptosis NADPH oxidase Xanthine oxidase Cyclooxygenase Electron transport chain |
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