超顺磁性氧化铁纳米粒子增强Jurkat T细胞的活力和增殖能力
投稿时间:2014-10-08  修订日期:2014-10-16  点此下载全文
引用本文:郝维,刘枭,代中华,曹济民.超顺磁性氧化铁纳米粒子增强Jurkat T细胞的活力和增殖能力[J].医学研究杂志,2015,44(3):23-27
DOI: 10.3969/j.issn.1673-548X.2015.03.008
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作者单位E-mail
郝维 100005 中国医学科学院基础医学研究所/北京协和医学院基础学院生理学系  
刘枭 100005 中国医学科学院基础医学研究所/北京协和医学院基础学院生理学系  
代中华 100005 中国医学科学院基础医学研究所/北京协和医学院基础学院生理学系  
曹济民 100005 中国医学科学院基础医学研究所/北京协和医学院基础学院生理学系 caojimin@126.com 
基金项目:国家重点基础研究发展计划("973"计划)项目(2011CB933500)
中文摘要:目的 观察超顺磁性氧化铁纳米粒子(SPION)对T淋巴细胞型白血病细胞株Jurkat E6-1细胞增殖和活力的影响。方法 常规培养Jurkat E6-1细胞, 将一定浓度的SPION加入经CFSE负载的Jurkat细胞中不同时间, 然后用PBS将细胞外的SPION洗去, 再换用完全培养基培养一定时间, 最后采用流式细胞术定量检测Jurkat细胞的增殖情况, 用CCK-8细胞活力测定试剂盒检测Jurkat细胞的活力。结果 SPION(20μg/ml)促进Jurkat细胞增殖, 使已分裂细胞百分比增高(与对照值相比, P<0.05), 分裂指数增高(与对照组相比, P<0.05);SPION也使Jurkat细胞的活力显著增强(与对照组相比, P<0.01)。此外, SPION对Jurkat细胞有钙动员作用, 可使Jurkat细胞的胞内游离钙水平显著增高。结论 SPION能促进Jurkat细胞增殖, 并增强其活力;这些效应与其钙动员作用有关。
中文关键词:超顺磁性氧化铁纳米粒子  Jurkat细胞  细胞增殖  钙动员
 
Superparamagnetic Iron Oxide Nanoparticles Enhance the Proliferation and Viability of Jurkat Cells
Abstract:Objective To investigate the effects of superparamagnetic iron oxide nanoparticles (SPION) on the proliferation and viability of Jurkat cells. Methods Jurkat E6-1 cells were conventionally cultured. SPION (20μg/ml) were added to CFSE-loaded Jurkat cells to act for different times, cells were then washed with PBS and were cultured for a period of time. Then, flow cytometry was used to quantitatively measure the proliferation, and CCK-8 method to evaluate the viability, of Jurkat E6-1 cells. Results SPION (20μg/ml) enhanced the proliferation of Jurkat cells, as shown by the increased percentage of devided Jurkat cells compared with that of the control (P<0.05) and by the increased division index (DI) of Jurkat cells (P<0.05 vs control). SPION also increased the viability of Jurkat cells (P<0.01 vs control). In addition, SPION exerted a calcium mobilizing effect on Jurkat cells, and it significantly increased the intracellular free Ca2+ level of Jurkat cells. Conclusion SPION enhances the proliferation and viability of Jurkat cells, and these effects are likely associated with its calcium mobilizing effect.
keywords:Superparamagnetic iron oxide nanoparticles  Jurkat cell  Cell proliferation  Calcium mobilization
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