| TLR2和NOD2在金黄色葡萄球菌肺炎小鼠中的表达 |
| 投稿时间：2014-08-07 修订日期：2014-09-09 点此下载全文 |
| 引用本文：温顺航,吴沪军,郭书真,刘榴,林罗娜,林立,李昌崇.TLR2和NOD2在金黄色葡萄球菌肺炎小鼠中的表达[J].医学研究杂志,2015,44(3):71-74 |
| DOI：
10.3969/j.issn.1673-548X.2015.03.020 |
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| 基金项目:浙江省自然科学基金重点项目(LZ13H010001);浙江省研究生创新科研项目(YK2011083) |
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| 中文摘要:目的 观察小鼠肺部感染金黄色葡萄球菌后肺组织模式识别受体TLR2及NOD2的表达, 探讨其在金黄色葡萄球菌肺炎中的作用。方法 30只C57BL/6J小鼠, 随机分为两组, 对照组小鼠滴鼻接种无菌磷酸缓冲盐溶液, 金黄色葡萄球菌肺炎组小鼠滴鼻接种5×108 CFU/50μl。滴鼻接种72h后, 获取肺组织标本, HE染色观察病理形态学改变, 免疫组化和Western blot法检测TLR2及NOD2蛋白的表达变化, QRT-PCR法检测TLR2和NOD2 mRNA的表达。结果 金黄色葡萄球菌滴鼻接种72h后, 小鼠肺组织TLR2和NOD2蛋白表达水平显著升高(P<0.05), 并且上调TLR2和NOD2 mRNA表达(P<0.01)。结论 金黄色葡萄球菌感染能上调小鼠肺组织TLR2和NOD2的mRNA及蛋白表达。 |
| 中文关键词:金黄色葡萄球菌 肺炎 Toll样受体2 核苷酸结合寡聚化结构域2 |
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| Expression of TLR2 and NOD2 in Pulmonary Tissue of Mice with Staphylocoocus aureus Pneumonia |
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| Abstract:Objective To investigate the expression of TLR2 and NOD2 in pulmonary tissue and its significance in mice with S. aureus pneumonia. Methods Thirty C57BL/6J mice were randomly divided into normal control and S. aureus pneumonia groups. Mice of the control group inoculated intranasally with sterile phosphate-buffered saline, and mice of the S. aureus pneumonia group inoculated intranasally with 5 × 108 CFU/50μl. Pulmonary tissues were collected at 72h after nasal inoculation. Changes in pulmonary histopathology were observed with HE staining, and the expression of TLR2 and NOD2 was analysed by immunohistochemistry and Western blot.QRT-PCR was used to detect TLR2 mRNA and NOD2 mRNA. Results At 72h after S. aureus inoculated, the expression of TLR2 and NOD2 protein in pulmonary tissues of mice were significantly increased (P<0.05, respectively), and the expression of TLR2 mRNA and NOD2 mRNA were elevated (P<0.01, respectively). Conclusion S. aureus infection can elevate the expression of TLR2 and NOD2 in mice pulmonary tissues. |
| keywords:Staphylocoocus aureus Pneumonia TLR2 NOD2 |
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