| LPS对5-FU杀伤QBC939细胞影响的实验研究 |
| 投稿时间：2014-08-17 修订日期：2014-09-16 点此下载全文 |
| 引用本文：霍胜军,吕忠诚,邹晓鹤,宋建文,王康.LPS对5-FU杀伤QBC939细胞影响的实验研究[J].医学研究杂志,2015,44(3):78-80 |
| DOI：
10.3969/j.issn.1673-548X.2015.03.022 |
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| 基金项目:江西省卫生厅基金资助项目(20091198) |
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| 中文摘要:目的 探讨LPS联合化疗药物5-FU对人胆管癌QBC939细胞生长和诱导细胞凋亡的影响。方法 四甲基唾哇蓝(MTT)试验检测LPS对5-FU抑制QBC939细胞增殖的影响, 流式细胞术观察LPS对5-FU诱导QBC939细胞凋亡的影响。结果 1MTT法检测显示5-FU对胆管癌细胞株QBC939杀伤作用明显, 但易出现耐药性, LPS可以增强5-FU后期杀伤作用, 能一定程度上防止耐药的出现;2流式细胞术结果显示化疗药物5-FU后能显著诱导QBC939细胞发生凋亡, 凋亡指数为11.50±2.15(P<0.05), 对照组为3.53±0.32, 加用LPS后, 能更明显地诱导凋亡, 凋亡指数达到26.50±3.12, 与单用加化疗药物5-FU比较, 差异有统计学意义(P<0.05)。结论 LPS能增强5-FU对胆管癌细胞株QBC939的后期杀伤作用, LPS联合化疗药物可能是胆管癌临床治疗的一种合理策略。 |
| 中文关键词:胆管癌QBC939 CXCR4 脂多糖 基因治疗 |
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| Experimental Study of Effect of Combined with 5-FU on Killer Cells of QBC939 |
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| Abstract:Objective To explore the effect of LPS lombined with chemotherapy drugs 5-FU on QBC939 human cholangiocarcinoma cell growth and induced cell apoptosis. Methods Tetramethyl spit wow blue (MTT) assay was used to obserre that LPS inhibited QBC939 cell proliferation by 5-FU.Flow cytometry was used to study the LPS on 5-FU induced apoptosis QBC939. Results 1MTT assay showed that 5-FU QBC939 cells killing effect was obvious, but prone to drug resistance. LPS could enhance the killing effect of 5-FU late, to prevent the emergence of resistance to a certain extent.2Flow cytometry showed that the chemotherapy drug 5-FU could significantly induce apoptosis in cells QBC939. Apoptotic index was 11.50±2.15 (P<0.05), (the control group was 3.53±0.32).When plusing LPS, apoptosis was more obvious, and apoptotic index reached 26.50±3.12. Compared with chemotherapy alone plus 5-FU, the difference was statistically significant (P<0.05). Conclusion LPS can enhance the killing effect of 5-FU late cholangiocarcinoma cell line QBC939. LPS bile duct cancer chemotherapy drugs may be a reasonable clinical strategy. |
| keywords:Cholangiocarcinoma cell QBC939 CXCR4 Lipopolysaccharide Gene therapy |
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