| 人参皂苷Rg1对异氟醚诱导caspase-3活化的影响 |
| 投稿时间：2014-09-29 修订日期：2014-10-08 点此下载全文 |
| 引用本文：缪慧慧,甄宇,丁冠男,洪方晓,田鸣.人参皂苷Rg1对异氟醚诱导caspase-3活化的影响[J].医学研究杂志,2015,44(4):52-58 |
| DOI：
10.11969/j.issn.1673-548X.2015.04.015 |
| 摘要点击次数: 997 |
| 全文下载次数: 708 |
|
| 基金项目:北京市科技新星计划基金资助项目(Z131107000413044) |
|
| 中文摘要:目的 探讨人参皂苷Rg1对异氟醚诱导caspase-3活化的影响及其机制。 方法 通过培养人神经胶质瘤细胞(H4 naïve cell)和稳定转染APP基因的细胞(H4-APP cell),给予25μmol/L人参皂苷Rg1预处理12h或者24h后接受异氟醚麻醉。采用蛋白免疫印迹法检测细胞凋亡效应蛋白caspase-3活化程度,利用化学发光法检测5'-三磷酸腺苷(adenosine-5'-triphosphate,ATP)水平的变化,流式细胞仪观察线粒体通透性转运孔(mitochondrial permeability transition pore,mPTP)的开放程度。 结果 给予25μmol/L人参皂苷Rg1预处理12h,H4 naïve细胞及H4-APP细胞的caspase-3活化程度,ATP水平及mPTP开放程度均与异氟醚处理组相比,差异无统计学意义(P>0.05)。延长人参皂苷Rg1预处理时间至24h,H4 naïve细胞及H4-APP细胞的caspase-3活化程度,ATP水平及mPTP开放程度均与异氟醚处理组差异有统计学意义(P<0.05)。 结论 人参皂苷Rg1可能通过保护线粒体功能降低异氟醚诱导caspase-3的活化程度。 |
| 中文关键词:人参皂苷Rg1 异氟醚 神经毒性 线粒体功能 |
| |
| Ginsenoside Rg1 Attenuates Isoflurane-Induced Caspase-3 Activation |
|
|
| Abstract:Objective To explore the possible beneficial effects and mechanisms of ginsenoside Rg1 on isoflurane-induced caspase-3 activation. Methods We investigated the effects of 25μmol/L ginsenoside Rg1 with 12h or 24h pretreated on isoflurane-induced caspase-3 activation in H4 naïve and stably-transfected human amyloid precursor protein (APP) (H4-APP cells).For mitochondrial dysfunction,we assessed the mitochondrial permeability transition pore (mPTP) and adenosine-5'-triphosphate (ATP) levels.We employed Western blot analysis, chemiluminescence and flowcytometry. Results Pretreatment with 25μmol/L ginsenoside Rg1 for 12h did not attenuate the isoflurane-induced caspase-3 activation and mitochondrial dysfunction in H4 naïve or H4-APP cells,while pretreatment with 25μmol/L ginsenoside Rg1 for 24h attenuated the isoflurane-induced caspase-3 activation and mitochondrial dysfunction both in H4 naïve and H4-APP cells. Conclusion Ginsenoside Rg1 may ameliorate the isoflurane-induced caspase-3 activation by inhibiting mitochondrial dysfunction. |
| keywords:Ginsenoside Rg1 Isoflurane Neurotoxicity Mitochondrial dysfunction |
| 查看全文 查看/发表评论 下载PDF阅读器 |
