| miR-21在早、晚期肺癌及肺大疱患者血清中的表达及临床意义 |
| 投稿时间：2014-07-31 修订日期：2014-09-26 点此下载全文 |
| 引用本文：叶敏华,杨伟芳,杨海华,丁维军,朱敏,孙晓南.miR-21在早、晚期肺癌及肺大疱患者血清中的表达及临床意义[J].医学研究杂志,2015,44(4):100-102,106 |
| DOI：
10.11969/j.issn.1673-548X.2015.04.027 |
| 摘要点击次数: 987 |
| 全文下载次数: 698 |
|
| 基金项目:浙江省台州市科技计划项目(111KY0608) |
|
| 中文摘要:目的 探讨miR-21在早、晚期肺癌及肺大疱患者血清中的不同表达及其临床意义。方法 以早、晚期肺癌患者及肺大疱患者(每组各20例,共60例)治疗前血清作为研究对象,使用RT-PCR检测其血清中miR-21表达变化,ROC曲线评估其敏感度和特异性。 结果 早、晚期肺癌患者血清中miR-21表达均明显高于肺大疱患者(P=0.003和P=0.000),且晚期肺癌患者血清中miR-21表达要高于早期肺癌患者(P=0.012)。miR-21诊断早、晚期肺癌的敏感度和特异性分别为65%和85%(AUC=0.775)、95%和 85%(AUC=0.928);miR-21用于区别肺癌晚期与早期的敏感度、特异性分别为45%和95%(AUC=0.733)。 结论 人血清miR-21表达检测可能有助于肺癌的早期诊断及判断其分期。 |
| 中文关键词:miR-21 血清 肺癌 肺大疱 早期诊断 生物标志物 |
| |
| Expression and Clinical Significance of Serum MicroRNA-21 in Different Stage Lung Carcinoma and in Pulmonary Bulla Patients |
|
|
| Abstract:Objective To identify circulating miR-21 expression in human serum as a biomarker for early detection and stratification in lung cancer. Methods We analyzed serum levels of miR-21 in 20 lung cancer patients with stage Ⅰ, 20 patients with stage Ⅲ or Ⅳ, and 20 patients with pulmonary bulla. Blood samples were collected before surgery and therapy. Quantitative reverse transcription-polymerase chain reaction of circulating miR-21 was performed. Receiver operating characteristic analysis was used to evaluate the sensitivity and speci? city of serum miR-21. Results We found that serum miR-21 was consistently expressed in both early and advanced stage lung cancer patients while in advanced stage patients which were more significantly. miR-21 expressions were capable of separating early and advanced stage lung cancer patients from control groups with an area under the curve of 0.775 and 0.928, separately. Conclusion Serum miR-21 may represent a novel biomarker for early detection of lung cancer and a potentially useful biomarker for disease progression. |
| keywords:miR-21 Lung cancer Pulmonary bulla Early detection Biomarker |
| 查看全文 查看/发表评论 下载PDF阅读器 |
