| L-半胱氨酸和溶血卵磷脂(16:0)作为卵巢癌标志物的诊断价值 |
| 投稿时间：2014-09-03 修订日期：2014-10-16 点此下载全文 |
| 引用本文：周璐,吕杰强,吴乐.L-半胱氨酸和溶血卵磷脂(16:0)作为卵巢癌标志物的诊断价值[J].医学研究杂志,2015,44(4):114-116,119 |
| DOI：
10.11969/j.issn.1673-548X.2015.04.031 |
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| 中文摘要:目的 为克服卵巢癌(ovarian cancer,OVC)早期检测缺陷,考察L-半胱氨酸和溶血卵磷脂(16:0)在检测OVC中的应用。 方法 选取温州医科大学附属第一医院2012年1月~2014年1月OVC患者(OVC组)142例和健康体检患者(对照组)100例,提取患者标本,采用MALDI-TOF-MS检测质谱峰,主成分分析法分析获得具有较大权重的峰m/z,分析鉴定,考察筛选代谢物在两组之间的表达差异,与OVC病理分级的相关性。 结果 184.05和496.30m/z在两组患者中差异最为明显,经鉴定为LPC(16: 0)与HCA;OVC组HCA检出率和平均水平显著高于对照组(P<0.01);对照组183.96m/z和478.33m/z峰面积均高于OVC组(P<0.01);HCA与病理分离呈正相关(P<0.05);183.96m/z和478.33m/z峰面积与病理分离呈负相关(P<0.05)。 结论 L-半胱氨酸和溶血卵磷脂(16:0)在OVC发病中的机制尚不清楚,但其可作为OVC检测的血清学标志物。 |
| 中文关键词:L-半胱氨酸 溶血卵磷脂(16:0) 卵巢癌 诊断 |
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| Diagnostic Value of L-homocysteic Acid and Lysophosphatidylcholine as Markers of Ovarian Cancer |
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| Abstract:Objective To study whether L-cysteine and lysolecithin (16:0) could be as a serum markers in the detection of OVC for overcoming the OVC defects of early detection. Methods Totally 142 cases of healthy check-up patients (control group) 100 cases from First Affiliated Hospital of Wenzhou Medical University in January 2012 to January 2014 were extracted patient specimens. These patient specimens were used to detect by MALDI-TOF-MS mass spectrometer and obtain the peak m/z by the method of principal component analysis for screening expression difference between the two groups in the metabolites and the correlation between metabolites and pathological grade. Results The most difference substance between two groups were 184.05 and 496.30m/z which identified as LPC (16:0) and HCA; On HCA average level and detection rate, OVC group was significantly higher than control(P<0.01); On 184.05 and 496.30m/z peak area, control group was significantly higher than OVC group(P<0.01); HCA positively was correlated with pathologic grade (P<0.05); 184.05 and 496.30 m/z peak area were negative correlation with pathologic grade (P<0.05). Conclusion L-cysteine and lysolecithin (16:0) mechanism in the pathogenesis of OVC is unclear, but it can be used for the detection of serum OVC markers. |
| keywords:L-cysteine Lysolecithin (16:0) Ovarian cancer Diagnosis |
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