| 褪黑素通过抑制p38通路减少腹腔巨噬细胞NO和ROS的产生 |
| 投稿时间：2014-11-19 修订日期：2014-11-25 点此下载全文 |
| 引用本文：邵布勒,张雨雪,王恺婧,应斐,孙学成,吴建胜.褪黑素通过抑制p38通路减少腹腔巨噬细胞NO和ROS的产生[J].医学研究杂志,2015,44(5):63-67 |
| DOI：
10.11969/j.issn.1673-548X.2015.05.017 |
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| 基金项目:浙江省自然科学基金资助项目(Q12H030005) |
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| 中文摘要:目的 探究褪黑素对脂多糖刺激后大鼠腹腔巨噬细胞产生的NO、ROS、p38及磷酸化p38的影响及其相关机制。方法 使用CCK8试验检测褪黑素对大鼠腹腔巨噬细胞的药物毒性;使用酶标仪检测NO的表达;使用荧光显微镜检测ROS的表达;RT-PCR法检测mRNA水平总p38的表达量;Western blot法检测p38 MAPK通路中p38以及磷酸化p38的表达量。结果 褪黑素对大鼠腹腔巨噬细胞无药物毒性。褪黑素及p38抑制剂(SB203580)均有效抑制了脂多糖刺激后大鼠腹腔巨噬细胞NO、ROS、以及磷酸化p38的表达,同时总p38的表达量并没有改变。结论 褪黑素通过抑制p38 MAPK(mitogen-activated protein kinase)信号通路的激活进而减少脂多糖刺激后大鼠腹腔巨噬细胞的NO(P=0.000)和ROS(P=0.000)的产生。 |
| 中文关键词:褪黑素 巨噬细胞 一氧化氮 活性氧簇 p38 |
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| Melatonin Decreases the Production of NO and ROS of Peritoneal Macrophages in Rat via Suppressing Activation of p38 |
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| Abstract:Objective To investigate the effect and mechanisms of melatonin on the production of nitric oxygen, reactive oxygen species(ROS),p38 and phosphorylated p38 of lipopolysaccharides (LPS) stimulated rat peritoneal macrophages. Methods The toxicity of melatonin was assessed by cell counting kit-8(CCK8) assay. ROS was detected by fluorescence microscope. The mRNA expression of total p38 was measured by reverse transcription-plolymerase chain reaction (RT-PCR) and the protein expression of p38 as well as phosphorylated p38 were detected by Western blot. Results Melatonin showed no toxicity on rat peritoneal macrophages. Melatonin along with p38 inhibitor(SB203580)both effectively suppressed the production of NO, ROS and the expression of phosphorylated p38 on LPS stimulated rat peritoneal macrophages, while dinot change the expression of total p38. Conclusion Melatonin reduce the production of NO (P=0.000) and ROS (P=0.000) of LPS stimulated rat peritoneal macrophages via inhibiting the activation of p38 MAPK signal pathway. |
| keywords:Melatonin Macrophage NO ROS p38 |
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