| 糖尿病肾病大鼠肾脏差异表达基因研究 |
| 投稿时间：2014-11-08 修订日期：2014-11-25 点此下载全文 |
| 引用本文：张茜,肖新华,黎明,李文慧,于淼,张化冰,平凡,杨国华.糖尿病肾病大鼠肾脏差异表达基因研究[J].医学研究杂志,2015,44(6):16-19 |
| DOI：
10.11969/j.issn.1673-548X.2015.06.006 |
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| 基金项目:国家临床重点专科建设项目(WDYZ2011-873); 国家自然科学基金资助项目(面上项目)(81170736);国家自然科学基金(青年基金)资助项目(81300649);中央高校基本科研业务费专项基金资助项目;中华医学会临床医学科研专项基金-默沙东糖尿病研究项目(12030450345);北京协和医院基金资助项目(2006119);北京协和医院中青年基金资助项目;协和青年基金资助项目(33320140022) |
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| 中文摘要:目的 糖尿病肾病是糖尿病患者的重要死亡原因。本研究旨在探讨糖尿病肾病大鼠肾脏差异表达基因,以期揭示DN发病的机制。方法 将5周龄SD大鼠随机分为正常组和糖尿病肾病模型组。糖尿病肾病模型组给予高脂饲料喂养加腹腔注射链脲佐菌素诱导大鼠糖尿病肾病模型。每4周测定空腹血糖、体重、24h尿白蛋白和尿肌酐水平。12周时处死大鼠,取血样测定血肌酐和血清尿素氮水平。取肾脏进行表达谱基因芯片实验。反转录实时定量PCR方法对芯片结果进行验证。结果 糖尿病肾病组空腹血糖,24h尿白蛋白、尿肌酐、血肌酐和尿素氮水平较正常组显著升高,而体重和尿肌酐显著降低。糖尿病肾病大鼠肾脏较正常组有624个基因差异表达,其中495个基因上调,129个基因下调。real-time PCR验证实验证实糖尿病肾病组ATP合成酶β亚基(Atp5b)、胶原蛋白1型α1(Col1α1)、细胞色素c氧化酶亚基Ⅵc(Cox6c)、NADH脱氢酶(辅酶Q)铁硫蛋白3(Ndufs3)和转化生长因子-β1(TGF-β1)显著上调。结论 氧化磷酸化通路和TGF-β1介导的细胞外基质(ECM)受体相互作用通路的激活可能与糖尿病肾病发生有关。 |
| 中文关键词:高血糖 糖尿病肾病 基因芯片 |
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| Gene Expression of Diabetic Nephropathy Rats in Kidney |
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| Abstract:Objective To investigate the gene expression of diabetic nephropathy (DN) rats in kidney. Methods SD rats were divided into control group and DN model group. Fasting blood glucose, body weight, 24h urinary albumin and urinary creatine were measured. On the 12th week, blood samples were taken to measure creatinine and usea nitrogen. Kidneys were taken to perform gene array. Real time PCR was performed to verify the result of gene array. Results Fasting blood glucose, 24h urinary albumin, urinary creatine, serum creatinine and usea nitrogen were significantly increased, body weight and urinary creatinine were significantly decreased in DN group. Kidney from diabetic rats had 624 genes with significantly changed expression (495 increased, 129 decreased). Real time PCR verified that ATP5b(F1-ATPase beta subunit), Col1α1 (collagen type 1 alpha 1), Cox6c (cytochrome c oxidase subunit Ⅵc), Ndufs3 (NADH dehydrogenase [ubiquinene] Fe-S protein 3) and TGF-β1 (transforming growth factor β1) increased in the DN model group. Conclusion Oxidative phosphorylation pathway, extracellular matrix (ECM) receptor interaction and TGF-β pathway may involved in the development of DN. |
| keywords:Hyperglycemia Diabetic nephropathy Gene array |
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