| SAK-HV蛋白通过上调ABCG5/ABCG8的表达降低胆固醇的吸收 |
| 投稿时间：2014-11-28 修订日期：2014-12-11 点此下载全文 |
| 引用本文：袁敏,王旻,付文亮,蔡贵玲,徐东刚.SAK-HV蛋白通过上调ABCG5/ABCG8的表达降低胆固醇的吸收[J].医学研究杂志,2015,44(7):17-22 |
| DOI：
10.11969/j.issn.1673-548X.2015.07.006 |
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| 基金项目:国家科技重大专项新药创制课题(2012ZX09102301-017);重大新药创制生物技术大平台(2012zx09301003-001-005-026) |
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| 中文摘要:目的 通过动物实验和体外细胞实验探讨SAK-HV蛋白降胆固醇的机制。方法 以0.5mg/kg浓度的SAK-HV蛋白治疗高脂喂养的ApoE-/-C57小鼠,酶法检测ApoE-/-C57小鼠血脂水平,定量PCR法(real-time quantitative PCR,qPCR)和蛋白质印迹(Western blot)法检测小肠ABCG5和ABCG8 mRNA和蛋白的表达水平。100μg/ml的SAK-HV蛋白作用caco-2细胞不同时间后,NBD胆固醇作为荧光探针检测SAK-HV蛋白对caco-2细胞胆固醇吸收的影响,q-PCR和Western blot法检测SAK-HV蛋白对caco-2细胞ABCG5和ABCG8 mRNA和蛋白表达水平的影响。结果 SAK-HV蛋白可以降低高脂喂养的ApoE-/-C57小鼠的血清胆固醇水平,同时上调小肠ABCG5和ABCG8 mRNA和蛋白的表达水平。体外实验表明,SAK-HV蛋白可以抑制caco-2细胞胆固醇的吸收,同时上调ABCG5和ABCG8 mRNA和蛋白的表达水平。结论 SAK-HV蛋白通过上调ABCG5和ABCG8的表达抑制小肠胆固醇的吸收从而降低血清胆固醇水平。 |
| 中文关键词:SAK-HV蛋白 ABCG5 ABCG8 小肠 胆固醇吸收 |
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| SAK-HV Protein Inhibits Cholesterol Absorption through Up-regulation of ABCG5/ABCG8 Expression |
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| Abstract:Objective To explore the mechanisms through that SAK-HV protein reduces cholesterol level. Methods We administrated high fat fed ApoE-/-C57 mice with SAK-HV protein of 0.5mg/kg concentration.Blood lipid levels were analysed in enzymic method. qPCR and Western blot were used to evaluate the expression of ABCG5 and ABCG8 in the small intestinal. To see whether the cholesterol absorption in the caco-2, cells was inhibited by SAK-HV protein.We pretreated the cells with SAK-HV protein of 100μg/ml concentration at different time followed by examination of the changes of cholesterol absorption in these SAK-HV-treated cells. Finally we determined whether SAK-HV protein affects the expression of ABCG5 and ABCG8 by both Western blot analysis and qPCR quantification.Results SAK-HV protein can reduce serum cholesterol levels of high fat fed ApoE-/-C57 mice and up-regulate the expression of ABCG5 and ABCG8 at both mRNA and protein level in the small intestine. The SAK-HV protein can inhibit the absorption of cholesterol in caco-2 cells and up-regulate the expression of ABCG5 and ABCG8 at both mRNA and protein level.Conclusion SAK-HV protein inhibits intestinal cholesterol absorption through up-regulation of ABCG5 and ABCG8 expression. |
| keywords:SAK-HV protein ABCG5 ABCG8 Small intestine Cholesterol absorption |
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