新型复方制剂斑晓胶囊对内皮祖细胞刺激活化的影响
投稿时间:2015-02-28  修订日期:2015-03-04  点此下载全文
引用本文:王贤,吴宗贵.新型复方制剂斑晓胶囊对内皮祖细胞刺激活化的影响[J].医学研究杂志,2015,44(7):33-36
DOI: 10.11969/j.issn.1673-548X.2015.07.010
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作者单位E-mail
王贤 200433 上海, 中国人民解放军第二军医大学附属长征医院心血管内科  
吴宗贵 200433 上海, 中国人民解放军第二军医大学附属长征医院心血管内科 wuzonggui12@163.com 
基金项目:国家自然科学基金资助项目(81130065)
中文摘要:目的 观察新型复方制剂斑晓胶囊对小鼠骨髓源性内皮祖细胞(endothelial progentor cells, EPCs)的刺激活化作用。方法 以不同浓度的斑晓胶囊制剂处理小鼠骨髓源性EPCs细胞后,用CCK-8法检测细胞增殖,采用流式细胞术的方法鉴定内皮祖细胞表面抗原CD-34、VEGFR-2的表达,采用Transwell法检测EPCs迁移功能,采用Western blot法检测Bcl-2/Bax蛋白水平的变化。结果 在所设加样量范围内,斑晓胶囊低剂量组和高剂量组与对照组相比,鼠源性EPCs的生长增殖能力增强(P<0.05),CD-34及VEGFR-2双阳性表达逐渐增多(P<0.05),Bcl-2蛋白水平上升,Bax蛋白水平下降,并呈浓度依赖性。结论 斑晓胶囊可促进小鼠骨髓源性EPCs的增殖,其机制可能与通过提高Bcl-2/Bax比值,抑制细胞凋亡有关。
中文关键词:复方制剂斑晓胶囊  小鼠骨髓源性内皮祖细胞  冠心病
 
Effects of New Compound Traditional Chinese Medicine Prepatations Banxiao Capsule on the Mobilization of Endothelial Progenitor Cells
Abstract:Objective To investigate the effects of new compound traditional Chinese medicine prepatations Banxiao capsule on the mobilization of endothelial progenitor cells (EPCs). Methods After EPCs from mouse bone marrow treated with variable dose of Banxiao capsule, we detected the proliferation by CCK-8 assay, quantified the number of CD34/Flk-1 double positive cells by flow cytometric analysis, evaluated the migratory function of EPCs by transwell chamber assay, and studied the protein level of Bcl-2/Bax by Western blot analysis. Results Compared with untreated group, the proliferation potential of EPCs in Banxiao low dose (20μg/ml) and high dose (200μg/ml) group was increased (P<0.05), the number of CD34/Flk-1 double positive cells were increased (P<0.05), migrative activity of EPCs was increased (P<0.05),the level of Bcl-2 protein were increased, and the level of Bax protein were decreased. Conclusion Banxiao capsule can enhance proliferation of EPCs through up-regulating of expression of Bcl-2/Bax.
keywords:Banxiao capsule  Endothelial progentor cells  Coronary heart disease
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