| 人脂肪干细胞复合富血小板血浆治疗裸鼠放射性皮肤损伤的实验研究 |
| 投稿时间：2014-12-04 修订日期：2014-12-12 点此下载全文 |
| 引用本文：张琳,富泽龙,冯锐.人脂肪干细胞复合富血小板血浆治疗裸鼠放射性皮肤损伤的实验研究[J].医学研究杂志,2015,44(7):57-61 |
| DOI：
10.11969/j.issn.1673-548X.2015.07.017 |
| 摘要点击次数: 914 |
| 全文下载次数: 734 |
|
| 基金项目:天津市卫生局科技基金资助项目(2011KR19) |
|
| 中文摘要:目的 以人脂肪干细胞(adipose-derived stem cells, ASCs)作为种子细胞,以富血小板血浆(platelet-rich plasma,PRP)作为载体制备复合物,观察其对裸鼠放射性皮肤损伤创面愈合的影响。方法 取吸脂术后健康成人的脂肪组织, 采用酶消化法分离培养ASCs,采用两步离心法制备富血小板血浆, 取第6代ASCs与PRP混合均匀,制备ASCs-PRP复合物。取24只裸鼠,随机分为4组(n=6),首先在裸鼠背部直径为2cm的圆形区域进行放射治疗(60Co,20Gy),然后在照射部位中央制备直径为1cm的皮肤全层缺损创面。模型完成后即刻,各组分别予以以下干预:ACSs+PRP组创面滴加ASCs-PRP复合物0.2ml(1×107个细胞/毫升), PRP组创面滴加PRP凝胶0.2ml,ASCs组创面滴加ASCs 0.2ml(1×107个细胞/毫升),对照组创面不加干预,直接覆盖敷料。术后7、14天各创面愈合情况,并绘制愈合曲线;术后第14天取材,行HE染色观察创面炎性反应及肉芽组织,免疫组织化学染色计数CD31阳性细胞的分布情况。结果 ASCs+PRP组创面第2周时完全愈合,对照组创面愈合最慢,第2周时仍以肉芽组织为主;ACSs、PRP组创面中央未能完全愈合。ASCs+PRP 组炎性细胞计数较ASCs组、PRP组及对照组明显减少,微血管计数CD31细胞阳性率明显高于其余3组,差异均有统计学意义(P<0.05)。结论 ASCs-PRP复合物可促进裸鼠放射性皮肤缺损微血管新生,促进创面愈合。 |
| 中文关键词:脂肪干细胞 富血小板血浆 放射性皮肤损伤 创面愈合 裸鼠 |
| |
| Effects of Human Adipose-derived Stem Cells-platelet-rich Plasma Composite on Healing of Radiation Skin Injury |
|
|
| Abstract:Objective To investigate the impact of human adipose-derived stem cells(hASCs)-plastelet-rich plasma(PRP) composite on the wound healing of radiation skin injury of nude mice. Methods ASCs were isolated from healthy adults after liposuction, conducted primary culture and subculture. PRP was prepared by two-step ultracentrifugation. The ASCs-PRP composite was prepared with ASCs (1×107cells/ml) at passage 6 and PRP. Twenty-four nude mice were randomly divided into 4 groups (n=6). A round shaped full-thickness skin defect (D=1cm) was made on the mice back after 20Gy (60Co) round shaped exposure (D=2cm). Right after radiation, wounds were treated as follows,in group A,there was ASCs+PRP, in group B, PRP,in group C, ASCs,in group D, sham group. The wounds were observed at postoperative 7 and 14 days. HE and CD31 stain were observed at 14th days after surgery. Results The wound healing of group A healed completely at 14th days. In group D,the wound healing were slowest and still filled with granulation tissue at 14th days. The wound healing of group B and group C were faster than that of group D, but they haven't healed completely at 14th days. The inflammatory cells were significantly lower and the blood vessel density positive rate were significantly higher in ACSs+PRP group than in ACSs group,PRP group and sham group (P<0.05). Conclusion ASCs-PRP Composite can accelerate the wound healing of radiation skin injury by promoting wound angiogenesis. |
| keywords:Adipose-derived stem cells Platelet-rich plasma Radiation skin injury Wound healing Nude mouse |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
