| 两种不同形状碳酸钙微米颗粒对大鼠骨髓间充质干细胞增殖及成骨分化影响的研究 |
| 投稿时间：2014-10-29 修订日期：2014-12-19 点此下载全文 |
| 引用本文：谷子芽,李颖,傅婷,张文元,杨亚冬,张科技.两种不同形状碳酸钙微米颗粒对大鼠骨髓间充质干细胞增殖及成骨分化影响的研究[J].医学研究杂志,2015,44(7):70-74 |
| DOI：
10.11969/j.issn.1673-548X.2015.07.020 |
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| 基金项目:浙江省科技厅基金资助项目(2011F20015);浙江省医药卫生科技计划项目(2014KYA040);杭州市科技发展计划项目(20130633B35) |
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| 中文摘要:目的 评价球形和方形两种碳酸钙微米粒对大鼠骨髓来源间充质干细胞增殖、分化等的影响。方法 制备球形和方形碳酸钙微粒并与大鼠骨髓间充质干细胞共培养,通过MTS法检测微粒对细胞增殖的影响,同时检测微粒在细胞表面的吸附以及引起的ROS反应,评价微粒的细胞毒性。通过检测碱性磷酸酶活性评价两种碳酸钙微粒对细胞成骨分化的影响。结果 两种碳酸钙微粒高浓度下造成一定细胞增殖抑制,方形微粒抑制作用更显著。微粒的细胞增殖抑制主要由微粒与细胞表面接触引起,但两种微粒均不引起细胞ROS水平升高。同时两种微粒高浓度下也可通过抑制碱性磷酸酶活性抑制成骨分化,方形微粒同样更明显的抑制碱性磷酸酶活性。结论 高浓度下碳酸钙微粒可对骨髓间充质干细胞增殖和成骨分化造成抑制,方形碳酸钙微粒具有更明显抑制现象,生物相容性不如等粒径球形微粒。因此球形微粒是组织工程支架材料更理想的选择。 |
| 中文关键词:骨髓间充质干细胞 碳酸钙 形状 细胞增殖 成骨分化 |
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| Effect of Calcium Carbonate Micro-particles with Different Shape on Cell Proliferation and Osteogenic Differentiation of Rat Bone Marrow Mesenchymal Stem Cells |
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| Abstract:Objective To evaluate the effect of calcium carbonate (CaCO3) micro-particles of different shapes (sphere and cubic) on proliferation and osteogenic differentiation of rat mesenchymal stem cells (MSCs) derived from bone marrow. Methods The MSCs were cocultured with CaCO3 micro-particles and cell proliferation was examined with MTS method. ROS and micro-particles attachment to the cells were also examined to evaluate cell toxicity. Alkaline phosphatase (ALP) activity were detected to evaluate osteogenic differentiation affected by calcium carbonate micro-particles. Results Both kinds of CaCO3 micro-particles can inhibit cell proliferation, although CaCO3 with cubic shape had more significant effect. This inhibition may be due to a direct contact between cells and the micro-particles. However, these CaCO3 micro-particles couldn't cause an increase in ROS level. Additionally, the two micro-particles inhibited osteogenic differentiation under high concentration by reducing ALP activity. Conclusion CaCO3 micro-particles can inhibit cell proliferation and osteogenic differentiation under high concentration. Cubic shaped micro-particles display more severe inhibition than sphere shaped ones, which indicate poorer biocompatibility. Therefore, CaCO3 micro-particles with sphere shape are more ideal option for tissue engineering. |
| keywords:Bone marrow mesenchymal stem cells Calcium carbonate Shape Cell proliferation Osteogenic differentiation |
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