| 母乳对Caco-2细胞缺氧复氧损伤模型NF-κB p65表达的影响 |
| 投稿时间：2014-12-12 修订日期：2015-01-07 点此下载全文 |
| 引用本文：陆玲,王宇军,冯伟伟,毕明远,阮为勇.母乳对Caco-2细胞缺氧复氧损伤模型NF-κB p65表达的影响[J].医学研究杂志,2015,44(7):135-138 |
| DOI：
10.11969/j.issn.1673-548X.2015.07.038 |
| 摘要点击次数: 1162 |
| 全文下载次数: 1037 |
|
| 基金项目:江苏省中西医结合医院基金资助项目(RC1102) |
|
| 中文摘要:目的 通过研究母乳对Caco-2细胞缺氧复氧损伤模型NF-κB p65表达的影响,以期探讨母乳对坏死性小肠结肠炎(necrotizing enterocolitis, NEC)的作用机制。方法 实验分为对照组和缺氧复氧损伤模型组,两组各有常规培养液组(MEM)、未加热乳清组(5%BM)和加热乳清组(5%BMb)。造模后向两组分别加入常规培养液、未加热乳清、加热乳清,继续培养6h后分别用RT-PCR检测法和免疫荧光法检测各组细胞中NF-κB p65表达情况。结果 RT-PCR检测结果显示对照组和模型组中,5%乳清和5%加热乳清均能抑制NF-κB p65 mRNA的表达(P<0.01),模型组中5%乳清抑制NF-κB p65 mRNA表达的作用较5%加热乳清更明显(P<0.05);免疫荧光结果显示,缺氧复氧损伤后Caco-2细胞质内的NF-κB p65被激活进入细胞核表达,5%乳清可以抑制NF-κB p65入核表达,并且较加热乳清作用更明显。结论 新鲜母乳可通过抑制NF-κB p65的表达防治小肠上皮细胞缺血再灌注损伤,其可能是母乳防治新生儿坏死性小肠结肠炎机制之一。 |
| 中文关键词:母乳 坏死性小肠结肠炎 Caco-2细胞 NF-κB p65 |
| |
| Impact of Breast Milk on the Expression of NF-κB p65 in Caco-2 Cells Model with Hypoxia/Reoxygenation Injury |
|
|
| Abstract:Objective To study the mechanisms of breast milk in preventing necrotizing enterocolitis (NEC) through determining the impact of breast milk on the expression of NF-κB p65 in Caco-2 cells model with hypoxia/reoxygenation injury. Methods The study had control groups and model groups.They were divided into three groups(MEM,5%BM and 5%BMb).We added conventional culture fluid,5% breast milk supernatant fluid and 5% breast milk supernatant fluid boiled into the three groups respectively after the model was builded.All cells continually cultured six hours, then the expression of NF-κB p65 in Caco-2 cells were detected with RT-PCR and immunofluorescence method.Results RT-PCR method showed that in control groups and model groups 5% breast milk supernatant fluid boiled or unboiled could inhibit the expression of NF-κB p65 mRNA(P<0.01).Compared with 5% breast milk supernatant fluid boiled,the inhibitory effect of 5% breast milk supernatant fluid was better in model groups(P<0.05). Immunofluorescence method showed that the expression of NF-κB p65 in the cytoplasm of Caco-2 cells with hypoxia/reoxygenation injury was activated into the nucleus,which could be inhibited by 5% breast milk supernatant fluid boiled or unboiled,and 5% breast milk supernatant fluid was better.Conclusion Fresh breast milk can prevent intestinal epithelial cells from ischemia reperfusion injury by inhibiting the expression of NF-κB p65,which may be one of the mechanisms of breast milk preventing NEC. |
| keywords:Breast milk Necrotizing enterocolitis Caco-2 cells NF-κB p65 |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
