| 番茄红素对糖尿病大鼠肾脏RAS系统的调节作用 |
| 投稿时间：2015-01-15 修订日期：2015-01-19 点此下载全文 |
| 引用本文：陶琳,杨毅.番茄红素对糖尿病大鼠肾脏RAS系统的调节作用[J].医学研究杂志,2015,44(8):117-121 |
| DOI：
10.11969/j.issn.1673-548X.2015.08.034 |
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| 中文摘要:目的 研究番茄红素对糖尿病大鼠肾脏肾素-血管紧张素系统(RAS)的调节作用及其机制。 方法 选用12周龄的雄性瘦型及肥胖型Zucker大鼠(2型糖尿病大鼠模型)各16只,随机分为瘦大鼠+溶剂组,肥胖大鼠+溶剂组,瘦大鼠+番茄红素组及肥胖大鼠+番茄红素组。分别给予溶剂(蒸馏水)或番茄红素[40mg/(kg·d)]口服喂养6周。代谢笼分析各组大鼠24h尿液;ELISA测定各组大鼠血清氧化应激(MDA,GSH)水平变化;放射免疫法检测各组大鼠肾脏组织肾素(Renin)及血管紧张素Ⅱ(AngⅡ)的含量;分别采用qRT-PCR及 Western blot法检测各组大鼠肾脏组织AT1受体mRNA及蛋白的表达水平。 结果 与瘦大鼠相比,校正体重因素后,肥胖糖尿病大鼠24h基础尿钠排泄显著降低(P<0.05),同时其体内氧化应激水平明显增高,即MDA活性水平显著升高,而GSH活性水平显著降低(P<0.05)。肥胖糖尿病大鼠肾脏Renin及AngⅡ水平也较瘦大鼠明显升高(P<0.05)。此外,肾脏AT1受体的mRNA及蛋白表达水平在肥胖糖尿病大鼠肾脏也显著高于瘦大鼠(P<0.05)。通过给予番茄红素处理6周后,可明显改善肥胖糖尿病大鼠上述指标的变化,降低肾脏Renin、AngⅡ水平及肾脏AT1受体的表达(P<0.05),而番茄红素对瘦大鼠无相应作用。 结论 番茄红素通过降低氧化应激水平,下调肥胖糖尿病大鼠肾脏RAS系统活性,从而促进血压的降低。 |
| 中文关键词:RAS 糖尿病 番茄红素 高血压 肥胖 |
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| Effect of Lycopene on Renal Renin Angiotensin System in the Diabetic Rat |
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| Abstract:Objective To investigate the effect of lycopene on renal renin angiotensin system and its mechanism in the diabetic rat. Methods Twelve-week-old male lean and obese rats (model of 2 diabetic rats) in each group (n=16) were used in this study. The rats were randomly divided lean rats+vehicle, lean rats+ lycopene, obese+vehicle and obese lycopene. Control lean and obese rats received drinking water, and those in the treatment goup were provided with lycopene [40mg/(kg·d)] for a duration of 6 weeks. 24-hour urine were collected by placing rats in stainless steel metabolic cages. The levels of oxidative stress including MDA and GSH were measured by ELISA. The levels of renin and angiotension Ⅱ(AngⅡ) in renal cortex were measured by radioimmunassay. The mRNA and protein levels of AT1 receptors in renal cortex were detected by qRT-PCR and Westernblot, respectively. Results Compared with lean rats, after adjustment for body weight, the basic urinary sodium excretion was significantly lower in obese diabetic rats (P<0.05); the levels of oxidative stress were significantly higher in obese diabetic rats, including increased actvity of MDA and decreased actvity of GSH (P<0.05). The levels of renin and AngII in renal cortex were also significantly increased in obese diabetic rats than lean rats (P<0.05). Moreover, the mRNA and protein expressions levels of the AT1 receptor were also higher in obese rats than lean rats (P<0.05). Lycopene treatment for 6 weeks improved the above-mentioned abnormalities in obese diabetic rats, including decreasing the levels of renin and AngⅡ, and the expression of AT1 receptor in renal cortex (P<0.05), but not in lean rats. Conclusion Lycopene treatment could reduce oxidative stress and downregulate the actvity of RAS in the kidney, which is helpful to lower blood pressure of obese diabetic rats. |
| keywords:RAS Diabetes Lycopene Hypertension Obese |
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