高危HPVE6/E7mRNA对宫颈病变诊断价值的研究
投稿时间:2015-03-16  修订日期:2015-03-27  点此下载全文
引用本文:郑智,吕杰强.高危HPVE6/E7mRNA对宫颈病变诊断价值的研究[J].医学研究杂志,2015,44(8):125-128
DOI: 10.11969/j.issn.1673-548X.2015.08.036
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作者单位E-mail
郑智 325000 温州医科大学第三临床学院、温州市人民医院妇产科  
吕杰强 温州医科大学附属第二医院妇产科 jieqianglu@126.com 
中文摘要:目的 探讨高危HPV E6/E7 mRNA对宫颈病变的临床诊断价值。 方法 根据病理诊断结果的不同分为两组,其中慢性宫颈炎、宫颈上皮内瘤变(CIN)Ⅰ级患者共48例为对照组,高级别宫颈病变(包括CINⅡ级、CINⅢ级及宫颈浸润癌)共42例患者为观察组,分别对上述宫颈细胞标本进行HPV DNA分型检测和高危HPV E6/E7 mRNA检测。 结果 慢性宫颈炎、CINⅠ组中高危HPV DNA的检出率(29.1%)明显高于高危HPV E6/E7 mRNA(8.3%),高级别宫颈病变组中高危HPV E6/E7 mRNA的检出率(85.7%)明显高于高危HPV DNA(64.3%),差异有统计学意义(P<0.05);高危HPV E6/E7 mRNA检测的敏感度、特异性、阳性预测值、阴性预测值(85.7%、91.7%、90.0%、88.0%)均高于HPV DNA分型检测(64.3%、70.8%、65.9%、69.3%),差异有统计学意义(P<0.05)。 结论 高危HPV E6/E7 mRNA检测在临床诊断过程中大大降低过度检查和治疗的机会,避免了高频率和重复感染给患者带来的经济负担和精神压力。
中文关键词:HPV  E6/E7  mRNA  HPV  DNA  宫颈癌
 
Study of High-risk HPV E6/E7 mRNA in Diagnosis of Cervical Lesions
Abstract:Objective To investigate the clinical diagnostic value of high-risk HPV E6/E7 mRNA in cervical lesions. Methods According to the different pathological diagnosis patients were divided into two groups. Totally 48 women who got chronic cervicitis or cervical intraepithelial neoplasia (CIN) level Ⅰ were in the control group,and totally 42 women who got high-grade cervical lesions (including CIN level Ⅱ, CIN level Ⅲ and invasive cervical cancer)were in the experimental group. Respectively, HPV DNA genotyping detection and high-risk HPV E6/E7 mRNA detection were perfomed on those cervical cell samples. Results The detection rate of high-risk HPV DNA (29.1%) was significantly higher than that of high-risk HPV E6/E7 mRNA (8.3%) in the chronic cervicitis,CIN I grade. The detection rate of high-risk HPV E6/E7 mRNA (85.7%) was significantly higher than that of high-risk HPV DNA (64.3%)in the high-grade cervical lesions grade, and the difference was significant (P<0.05).The sensitivity,specificity,positive predictive value and negative predictive value of high-risk mRNA detection(85.7%,91.7%,90.0%,88.0%)were higher than those of HPV DNA genotyping detection(64.3%,70.8%,65.9%,69.3%),and the difference was statistically significant(P<0.05). Conclusion The detection of high-risk HPV E6/E7 mRNA as a means of present commonly used diagnostic methods, combining the transcriptional activity of cancer gene diagnosis can be in the diagnostic process, which greatly reduces the excessive examination and treatment opportunity, avoids the short term high frequency and repeated infection brings to the patient's economic burden and mental pressure.
keywords:HPV E6/E7 mRNA  HPV DNA  Cervical cancer
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