| Aurora-A在食管鳞癌及癌前病变中的表达特点和研究意义 |
| 投稿时间：2015-04-22 修订日期：2015-05-05 点此下载全文 |
| 引用本文：韩旭,薛丽燕,沈笑,程贤,詹启敏,童彤.Aurora-A在食管鳞癌及癌前病变中的表达特点和研究意义[J].医学研究杂志,2015,44(9):21-26 |
| DOI：
10.11969/j.issn.1673-548X.2015.09.007 |
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| 基金项目:国家重大科学研究计划基金资助项目(2010CB910703) |
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| 中文摘要:目的 探讨极光激酶(aurora kinases A,Aurora-A)在食管鳞癌及癌前病变(主要为癌旁癌前病变)组织样本中的表达特征及其在食管鳞癌发生、发展过程中的作用。 方法 应用组织芯片技术和免疫组织化学方法(S-P法)检测Aurora-A在9例重度不典型增生组织和122例食管鳞癌组织以及它们的癌旁癌前病变组织中的表达情况;并使用实时荧光定量PCR法检测Aurora-A基因在8对癌前病变组织和5对早期食管癌组织中的表达水平;同时运用蛋白免疫印迹法和实时荧光定量PCR法检测Aurora-A基因在各细胞株(人食管上皮永生化细胞株及食管鳞癌细胞株)中的差异性表达情况。 结果 免疫组化结果显示Aurora-A在正常食管黏膜上皮、轻度不典型增生、中度不典型增生、重度不典型增生及食管鳞癌组织中的阳性率分别为17.2%、27.6%、50.0%、71.2%和84.3%,其表达率随病变程度加重而递增。Aurora-A基因mRNA水平在8例癌前病变组织和5例早期食管癌组织中的表达程度也明显高于其相应正常组织,其中病变组织存在Aurora-A显著性高表达的比率分别为75.0%(6/8)和60.0%(3/5)。与人食管上皮永生化细胞株相比,Aurora-A在ESCC细胞株中的表达水平也明显升高。 结论 Aurora-A激酶表达水平与食管癌癌前病变严重程度呈正相关(r=0.548,P=0.000),提示Aurora-A可能参与食管鳞癌的发生与发展,有望为食管鳞癌的早期诊断及治疗提供新的方向。 |
| 中文关键词:Aurora-A 食管癌 癌前病变 |
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| Study on the Expression and Significance of Aurora-A in Esophageal Squamous Cell Carcinoma and Precancerous Lesions |
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| Abstract:Objective To investigate the expression and function of Aurora Kinases A(Aurora-A) in esophageal squamous cell carcinoma (ESCC) and its precursor lesions (most of which are tissues adjacent to carcinoma). Methods We used tissue microarray and immunohistochemistry to detect the expression patterns of Aurora-A in 9 cases of severe dysplasia and 122 cases of ESCC combined with adjacent precursor lesions. The expression of Aurora-A in 8 cases of precancerous tissues and 5 cases of early esophageal cancer tissues were detected by Real-time PCR. The expression of Aurora-A in different cell lines (immortalized esophageal epithelium cell lines and ESCC cell lines) were detected by Western blotting and Real-time PCR. Results The positive rate of Aurora-A in normal esophageal epithelium, mild dysplasia, moderate dysplasia, severe dysplasia and ESCC was 17.2%, 27.6%, 50.0%, 71.2% and 84.3%, separately. A progressive increase was along with the grade of the lesions. At the mRNA level, Aurora-A also expressed higher in 8 cases of precancerous tissues and 5 cases of early esophageal cancer tissues than in normal tissues with a significantly rate of 75.0% (6/8) and 60.0% (3/5), respectively. Compared with immortalized esophageal epithelium cell lines, Aurora-A was significantly overexpressed in ESCC cell lines. Conclusion Aurora-A expression was up-regulated with the increasing grade of esophageal lesion (r=0.548, P=0.000), which indicated Aurora-A could be involved in the development and progression of ESCC. Aurora-A might provide a new way for early diagnosis and treatment of ESCC in the future. |
| keywords:Aurora-A Esophageal squamous cell carcinoma Precancerous lesions |
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