| 叶酸受体-α、Legumain在视网膜母细胞瘤细胞系的表达实验研究 |
| 投稿时间：2015-03-14 修订日期：2015-03-30 点此下载全文 |
| 引用本文：黄平,翁郁华,孟永春,南开辉,吕帆.叶酸受体-α、Legumain在视网膜母细胞瘤细胞系的表达实验研究[J].医学研究杂志,2015,44(9):47-51 |
| DOI：
10.11969/j.issn.1673-548X.2015.09.013 |
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| 基金项目:国家国际科技合作专项基金资助项目(2012DFB30020);浙江省自然科学基金资助项目(LY12H12005);温州市科技计划项目(Y20130115) |
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| 中文摘要:目的 研究视网膜母细胞瘤中叶酸受体-α(FR-α)和天冬氨酸内肽酶(Legumain)的表达,以视网膜母细胞瘤细胞系Y-79为研究对象,通过与正常视网膜色素上皮细胞ARPE-19对比,揭示瘤细胞中两种细胞因子的表达水平。 方法 应用实时荧光定量PCR(Q-PCR)法检测Y-79、ARPE-19细胞叶酸受体和Legumain的mRNA表达水平;运用Western blot法检测叶酸受体和Legumain的蛋白表达水平。结果 Y-79细胞中FR和Legumain的mRNA表达水平分别是ARPE-19的4倍和6.47倍;Western blot法检测结果表明FR-α在Y-79细胞的蛋白表达水平比ARPE-19细胞高1.35倍,两者有显著性差异(P<0.05);Legumain在两种细胞中均以36kDa的成熟酶形式表达,Y-79细胞中的表达水平比ARPE-19细胞高出3倍,且有显著性差异。 结论 与正常组织细胞系——视网膜色素上皮细胞ARPE-19相比,叶酸受体FR-α和Legumain在视网膜母细胞瘤细胞系中表达要高。 |
| 中文关键词:视网膜母细胞瘤 叶酸受体 Legumain 实时荧光定量PCR |
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| Expression of Folate Receptor-alpha and Legumain in Retinoblastoma Cell Line |
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| Abstract:Objective To investigate the expression of folate receptor-alpha and Legumain in retinoblastoma. We compared the differential expression of retinoblastoma cell line Y-79 and retinal pigment epithelial ARPE-19 cells in both the mRNA and protein levels. Methods In our study, the mRNA expression of FR-α and Legumain in two types of cells was detected by Q-PCR. Furthermore, the protein expression level of FR-α and Legumain were also detected by Western blot. Results The mRNA expression of FR-α and Legumain in Y-79 cells were 4 and 6.47 times higher than that in ARPE-19, respectively, which indicated a higher genetic expression of two cytokines in cancer cells. The results of Western blot showed that, the FR-α protein in Y-79 cells was 1.35 times more compared with the ARPE-19 cells, and there was a significantly statistical differences (P<0.05). Legumain existed in the form of 36kDa mature enzyme in both cells, and the expression of Legumain protein in Y-79 was 3 times higher than ARPE-19. Conclusion Both FR-α and Legumain were overexpressed in RB cell line Y-79 compared to normal cells ARPE-19. |
| keywords:Retinoblastoma Folate receptor Legumain Q-PCR |
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