| ALK7基因敲除对小鼠心电图的影响及可能机制 |
| 投稿时间：2015-04-22 修订日期：2015-05-13 点此下载全文 |
| 引用本文：余太辉,胡晓军,王鑫,陈卉,李乐,唐艳红.ALK7基因敲除对小鼠心电图的影响及可能机制[J].医学研究杂志,2015,44(10):24-28 |
| DOI：
10.11969/j.issn.1673-548X.2015.10.008 |
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| 基金项目:国家自然科学基金资助项目(81370282) |
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| 中文摘要:目的 探讨ALK7基因敲除对小鼠心电图的影响及可能机制。 方法 采用8~10周的C57BL/6的野生型小鼠以及ALK7 基因敲除小鼠,使用自制皮下电极连接Powerlab系统记录9:30~10时小鼠Ⅱ导联心电图,使用Labchart软件直接分析RR间期、HR、PR间期、P波宽度、QRS宽度、QT间期以及QTc,并进一步免疫组化检测PPAR-γ的表达。 结果 与正常野生型小鼠相比,ALK7基因敲除小鼠的RR间期、HR、P波脉宽、PR间期、QRS间期无明显差异(P>0.05),然而QT间期明显延长,QTc明显增大(P<0.05)。免疫组化检测ALK7基因敲除小鼠PPAR-γ的阳性表达率显著减少(P<0.05)。 结论 ALK7基因敲除小鼠PPAR-γ的减少可能影响小鼠心室复极变化,出现QT间期的延长。 |
| 中文关键词:ALK7 QT延长 复极异常 |
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| ECG Characteristics in the ALK7-Null Mice and the Possible Mechanisms |
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| Abstract:Objective To elucidate the effects of activin receptor-like kinase 7(ALK7) in ECG, and possible mechanism. Methods Male wildtype and ALK7-/- mice (C57BL/6 background) aged 8 to 10 weeks were used in the studies. ECG (Lead Ⅱ) was continuously recorded in consciously moving mice by homemade subcutaneous electrode from 9:30 to 10.The P-wave duration and, RR interval, PR interval and QRS duration,QT interval and QTc were measured with the Labchart software.In addition, expression of PPAR-γ were analysed by immunohistochemistry. Results There was no significant difference in P-wave duration,RR interval,PR interval and QRS duration between wildtype and ALK7-/- mice.But QT interval and QTc of ALK7-/- mice were prolonged obviously compared with wildtype mice.In addition,positive expression rate of PPAR-γ in ALK7-/- mice was significantly reduced compared with wildtype mice. Conclusion The abnormal ventricular repolarization in ALK7-/- mice may be due to reduce in expression of PPAR-γ, and results in prolonged obviously of QT interval. |
| keywords:ALK7-/- mice QT interval Abnormal repolarization |
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