| 可梯度降解支架细胞化后修复兔前交叉韧带缺损 |
| 投稿时间：2015-02-02 修订日期：2015-02-13 点此下载全文 |
| 引用本文：张文元,杨亚冬,张科技,唐靓,房国坚.可梯度降解支架细胞化后修复兔前交叉韧带缺损[J].医学研究杂志,2015,44(10):38-41 |
| DOI：
10.11969/j.issn.1673-548X.2015.10.011 |
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| 基金项目:浙江省自然科学基金资助项目(LY13H060004),浙江省医学重点学科群项目生物医药重点学科群(XKQ-010-001);浙江省医药卫生科技计划项目(2014ZDA005,2013KYA045) |
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| 中文摘要:目的 通过可梯度降解的绳-网支架复合自体骨髓间充质干细胞(MSCs),修复兔前交叉韧带(ACL)缺损。 方法 将聚乳酸: 蚕丝丝素: 聚羟基乙酸纤维按质量比7: 6: 5混合,分别捻拧成绳芯与纬编针织成网状物,滴加Ⅰ型胶原并冻干。将网状物包裹绳芯,种植兔自体MSCs,植入兔前交叉韧带(ACL)缺损处,作为实验组。单纯支架置入为对照组。分别于术后12、24周,取材,HE染色,并行力学测试。 结果 术后12周,实验组新生韧带与骨之间产生大量有序的垂直胶原纤维;对照组形成少量垂直胶原纤维。术后24周,实验组形成类似直接止点结构;对照组则产生许多垂直胶原纤维。力学测试结果显示,术后12、24周实验组的最大载荷分别为72.7±23.4N与121.8±34.3N,与对照组比较,差异均有统计学意义(P<0.01)。 结论 可梯度降解支架复合自体MSCs能较好地重建兔ACL。 |
| 中文关键词:前交叉韧带 修复 骨髓间充质干细胞 梯度降解支架 |
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| Gradient Degraded Scaffold Seeded with Mesenchymal Stem Cells Repairing Anterior Cruciate Ligament in Rabbits |
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| Abstract:Objective To use a gradient degraded wire-mesh scaffold compound autologous bone marrow-derived mesenchymal stem cells (MSCs) repairing rabbit anterior cruciate ligament(ACL) defect. Methods After polylactic acid(PLA): silk fibroin(SF): polylactic acid(PGA) fibers(mass ratio 7: 6: 5) being mixed, they were twisted into a rope core and knitted into mesh respectively, then the rope core was wrapped with the mesh to obtain wire-mesh binding scaffold.Autologous MSCs were seeded in the binding scaffold to produce scaffold-MSCs complexe.The complexe was implanted in defect of ACL in rabbits, as experimental group.Only scaffold was implanted, as control group.12 and 24 weeks postoperatively,the animals were sacrificed for HE staining of longitudinal sections of neo-ACL together with tibia or femur and biomechanical testing of newborn ACL-bone. Results Twelve weeks postoperatively, many Sharpey's fibers were generated to form indirect stop point in experimental group. While in control group,cells lineage was disorder, and rare Sharpey's fibers were formed.Twenty four weeks postoperatively,a structure similar to direct stop point of four areas from newborn ACL to bone was formed in experimental group.While in control group, many Sharpey's fibers were generated to form indirect stop point.Biomechanical testing of newborn ACL-bone results showed that: 12 and 24 weeks postoperatively, the maximum load of newborn ACL-bone in experimental group were 72.7±23.4N and 121.8±34.3N,respectively,and there were statistical significance(P<0.01) compared with the control group. Conclusion The gradient degraded wire-mesh scaffold seeded with MSCs can make ACL defect get a well repair. |
| keywords:Anterior cruciate ligament Repair Mesenchymal stem cells Gradient degraded scaffold |
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