| 绝经对大鼠血栓前状态的影响 |
| 投稿时间：2015-05-19 修订日期：2015-06-01 点此下载全文 |
| 引用本文：高辉,奚水,徐苓.绝经对大鼠血栓前状态的影响[J].医学研究杂志,2015,44(10):89-92 |
| DOI：
10.11969/j.issn.1673-548X.2015.10.025 |
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| 中文摘要:目的 通过去势建立绝经大鼠模型,观察绝经对大鼠血栓前状态的影响。 方法 将32只9~10月龄的健康雌性SD大鼠随机分成4组,每组8只。实验组手术去势,对照组行假手术,分别于术后1周或10周处死。采用凝血酶比浊法检测各组大鼠血浆纤维蛋白原(FIB)含量;采用发射底物法检测抗凝血酶Ⅲ(ATⅢ)活性;采用ELISA双抗体夹心法检测纤溶酶原激活剂抑制剂(PAI-1)、D-二聚体(D-D)、血管性假血友病因子(vWF)含量;采用硝酸还原酶法检测NO含量;采用放射性核素放射免疫法检测血浆血栓素A2(TXA2)、前列环素(PGI2)含量。 结果 在绝经早期,血浆FIB含量明显升高,血浆ATⅢ活性无明显变化;血浆PAI-1含量呈升高趋势;血浆vWF含量显著升高,同时血浆NO含量显著下降;随着绝经时间延长,到绝经晚期,与对照组相比,PAI-1含量显著增加,而血浆FIB含量有所回落,血浆ATⅢ活性显著升高;血浆vWF含量仍保持在较高水平,但血浆NO含量有所回升。在绝经早期及绝经晚期,血浆TXA2和PGI2同步升高,D-D含量各组间均无明显变化。 结论 在血栓形成的不同环节,绝经事件发挥了不同的影响,导致绝经早期血栓形成风险增加;随着绝经时间延长,各相关参数达成新的平衡,血栓风险回落。 |
| 中文关键词:血栓前状态 绝经 去势大鼠 |
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| Effects of Menopause on Prothrombotic State in Ovariectomized Rats |
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| Abstract:Objective To observe the effects of menopause on prothrombotic profiles in ovariectomized rats. Methods Thirty two healthy female SD rats of 9 to 10 months were divided into 4 groups, the control shamed and the observation groups ovariectomized. Rats in the baseline group and the early menopause group were sacrificed one week later, and the control and late menopause group 10 weeks later. The prothrombotic profiles were detected including plasma FIB, ATⅢ activity, PAI-1 levels, D-D level, vWF levels and NO concentration, TXA2 and PGI2 levels. Results In early menopause, plasma FIB increased dramatically while ATⅢ activity remained little changed. PAI-1 demonstrated an increasing trend. vWF significantly increased but NO significantly decreased. In later menopausal stage, PAI-1 increased dramatically but FIB somewhat decreased. Plasma ATⅢ activity significantly increased and vWF still remained a high level. NO increased a little. In both early and later stage, TXA2 and PGI2 simultaneously increased while D-D showed little change between groups. Conclusion Menopause plays different roles in different aspects of thromoembolism, resulting in increased risk in early menopause due to prothrombotic state and decreased risk in later menopause when new balances between profiles were established. |
| keywords:Prothrombotic state Menopause Ovariectomized rats |
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