| 白藜芦醇通过下调p-ERK1/2抑制脂多糖诱导的H9c2细胞损伤 |
| 投稿时间：2015-03-06 修订日期：2015-03-18 点此下载全文 |
| 引用本文：叶婷婷,王光宇,李丽,马建,魏盟.白藜芦醇通过下调p-ERK1/2抑制脂多糖诱导的H9c2细胞损伤[J].医学研究杂志,2015,44(10):110-113 |
| DOI：
10.11969/j.issn.1673-548X.2015.10.031 |
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| 中文摘要:目的 探讨白藜芦醇(resveratrol,RSV)是否通过下调p-ERK1/2 发挥对脂多糖(lipopolysaccharide,LPS)诱导的H9c细胞氧化损伤的保护作用及机制。 方法 将H9c2细胞分为实验(Con)组,脂多糖(LPS)组,脂多糖+白藜芦醇5μmol/L(L+R5)组,脂多糖+白藜芦醇10μmol/L(L+R10)组,脂多糖+白藜芦醇20μmol/L(L+R20)组,脂多糖+白藜芦醇50μmol/L(L+R50)组。其中对照组不做任何处理,L+R5组、L+R10组、L+R20组、L+R50组分别用5、10、20、50μmol/L的白藜芦醇预先处理24h,然后将LPS组、 L+R5组、L+R10组、L+R20组、L+R50组和10μg/ml的脂多糖共同孵育20min和12h。MTT比色法检测H9c2细胞活力,Western blot法检测 p-ERK1/2,ERK1/2蛋白表达。 结果 LPS降低H9c2细胞活力,诱导细胞凋亡。RSV 上调p-ERK1/2蛋白表达,成剂量依赖性。RSV 预处理能明显降低LPS对H9c2细胞的损伤。 结论 白藜芦醇可能通过下调p-ERK1/2 来减轻LPS引起的H9c2 细胞损伤,发挥其保护作用。 |
| 中文关键词:白藜芦醇 脂多糖 ERK1/2 H9c2细胞 |
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| Resveratrol Protects H9c2 Cells from Damage Induced by Lipopolysaccharide via Down-regulating the Phosphoration of ERK1/2 |
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| Abstract:Objective To investigate whether resveratrol(RSV)protects H9c2 cells against lipopolysaccharide (LPS) induced oxidative injury partly through ERK1/ 2 signaling pathway. Methods H9c2 cells were divided into six groups: control, LPS (10μg/ml lipopolysaccharide),L+R5(10μg/ml lipopolysaccharide+5 μmol/L resveratrol),L+R10(10μg/ml lipopolysaccharide+10μmol/L resveratrol),L+R20(10μg/ml lipopolysaccharide+20 μmol/L resveratrol),L+R50(10μg/ml lipopolysaccharide+50μmol/L resveratrol). H9c2 cells from group L+R5,L+R10,L+R20,L+R50 were pretreated with resveratrol.Then, cells from group LPS,L+R5,L+R10,L+R20,L+R50 were incubated with LPS for 20 min and 12 hours. MTT were used to detect cell proliferation. The protein level of ERK1/2, and phosphorylation of ERK1/2 was measured by Western blot respectively. Results Compared with control, LPS significantly reduced cell proliferation (P<0.05), increased(P< 0.01)cell death, and up-regulated the level of phosphorylation of ERK1/2(P<0.01). Pretreatment of resveratrol attenuated the inhibition of LPS on cell viability and down-regulated the level of phosphorylation of ERK1/2. Conclusion Resveratrol may exert its cytoprotection effects on LPS-induced H9c2 cells via down regulating the activation of p-ERK1/2. |
| keywords:Resveratrol Lipopolysaccharide H9c2 cardiomyocytes p-ERK1/2 |
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