| 二苯乙烯苷对低氧肺动脉平滑肌细胞增殖的影响 |
| 投稿时间：2015-02-05 修订日期：2015-02-26 点此下载全文 |
| 引用本文：张志勇,杨水珍.二苯乙烯苷对低氧肺动脉平滑肌细胞增殖的影响[J].医学研究杂志,2015,44(10):151-154 |
| DOI：
10.11969/j.issn.1673-548X.2015.10.043 |
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| 中文摘要:目的 探讨二苯乙烯苷对低氧肺动脉平滑肌细胞(PASMCs)增殖的影响及其机制。 方法 SD大鼠PASMCs经低氧及TSG干预24h后, CCK-8检测细胞增殖及TSG的细胞毒作用,流式细胞仪分析细胞周期,实时定量PCR(RT-PCR)检测HIF-1α mRNA的表达,酶标仪检测活性氧(ROS)的产生。 结果 二苯乙烯苷能够抑制低氧PASMCs增殖,阻滞细胞周期于G0/G1~S期,并且实验浓度的二苯乙烯苷无明显细胞毒作用,进一步的研究发现二苯乙烯苷能够抑制HIF-1α mRNA的表达及ROS的产生。 结论 二苯乙烯苷可抑制低氧PASMCs增殖,其机制可能与抑制HIF-1α mRNA的表达及ROS产生有关。 |
| 中文关键词:低氧 肺动脉平滑肌细胞 二苯乙烯苷 增殖 |
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| Effect of 2,3,4',5-Tetrahydroxystilbene-2-O-beta-D-glucoside on the Proliferation of Pulmonary Artery Smooth Muscle Cells Induced by Hypoxia |
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| Abstract:Objective To investigate the effects of the 2,3,4',5-Tetrahydroxystilbene-2-O-beta-D-glucoside on the proliferation of PASMCs induced by hypoxia, in orde to search new drugs for the treatment and prevention of hypoxic pulmonary vascular remodeling. Methods 3% O2 hypoxia was used to induced the proliferation of PASMCs. After hypoxic and TSG treatment for 24h, cell growth was determined by cell counting kit-8 (CCK-8), cell cycle was analysed by flow cytometry, the mRNA expression of HIF-1α was measured by quantitative real-time PCR, and the reactive oxygen species (ROS) production was determined by the fluorescence microplate reader. Results TSG can block the proliferation of PASMCs through G0/G1 to S phase of the cell cycle arrest without cell cytotoxicity. Further experiments showed that TSG blocking the proliferation of PASMCs was associated suppression the mRNA expression of HIF-1α and the production of intracellular ROS in hypoxia-stimulated-PASMCs. Conclusion TSG can inhibit the proliferation of pulmonary artery smooth muscle cells induced by hypoxia through suppression the mRNA expression of HIF-1α and the production of intracellular ROS. |
| keywords:Hypoxia Pulmonary artery smooth muscle cells 2,3,4',5-Tetrahydroxystilbene-2-O-beta-D-glucoside Proliferation |
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