miR-181b-5p对卡波西肉瘤细胞系SLK细胞迁移、侵袭的影响
投稿时间:2015-04-26  修订日期:2015-06-18  点此下载全文
引用本文:丁媛,梁俊琴,吴秀娟,向芳,康晓静,王红娟,普雄明.miR-181b-5p对卡波西肉瘤细胞系SLK细胞迁移、侵袭的影响[J].医学研究杂志,2015,44(11):35-39
DOI: 10.11969/j.issn.1673-548X.2015.11.010
摘要点击次数: 1005
全文下载次数: 864
作者单位E-mail
丁媛   
梁俊琴 830001 乌鲁木齐, 新疆维吾尔自治区人民医院皮肤性病科  
吴秀娟 830001 乌鲁木齐, 新疆维吾尔自治区人民医院皮肤性病科  
向芳 830001 乌鲁木齐, 新疆维吾尔自治区人民医院皮肤性病科  
康晓静 830001 乌鲁木齐, 新疆维吾尔自治区人民医院皮肤性病科  
王红娟 830001 乌鲁木齐, 新疆维吾尔自治区人民医院皮肤性病科  
普雄明 830001 乌鲁木齐, 新疆维吾尔自治区人民医院皮肤性病科 puxiongming@126.com 
基金项目:国家自然科学基金地区科学基金资助项目(81260311);新疆维吾尔自治区自然科学基金项目(2014211A059)
中文摘要:目的 通过脂质转染miR-181b-5p模拟物及抑制物到卡波西肉瘤细胞系SLK,研究miR-181b-5p对卡波西肉瘤细胞SLK细胞增殖、迁移、侵袭生物学功能的影响。方法 应用脂质体对人卡波西肉瘤细胞株SLK进行转染,转染miR-181b-5p模拟物及抑制物后应用MTT测定细胞增殖曲线,应用流式细胞仪行检测细胞周期。利用Transwell小室试验后行苏木素染色观察细胞迁移、细胞侵袭生物学特性。结果 miR-181b-5p模拟物促进细胞增殖,促进S期的转变,加速细胞周期进程;miR-181b-5p抑制物抑制细胞增殖,诱导细胞发生G0/G1期阻滞,延缓细胞周期进程。Transwell小室迁移实验结果显示,与阴性对照组迁移实验下室面细胞数目151±11个相比,miR-181b-5p模拟物组184±9个,细胞数目明显数量增多,迁移能力均明显提高,差异有统计学意义(P<0.05)。Transwell小室侵袭实验结果显示,与阴性对照组侵袭实验下室面细胞数目35±6个相比,miR-181b-5p模拟物组48±5个,细胞数目明显数量增多,侵袭能力均明显提高(P<0.05)。结论 miR-181b-5p在SLK细胞中高表达,其对SLK细胞的增殖、侵袭和迁移能力可能存在正向调控作用,可能成为卡波西肉瘤的潜在治疗靶点。
中文关键词:miR-181b-5p  卡波氏肉瘤  细胞周期  迁移  侵袭
 
Effects of microRNA-181b-5p on Migration and Invasion of Human Kaposi's Sarcoma Cell Line SLK.
Abstract:Objective miR-181b-5p mimics and inhibitors were transfected into Kaposi's sarcoma cell line SLK through liposomal transfection. To study the effect of miR-181b-5p on SLK cell proliferation,migration and invasion biological activities. Methods Human Kaposi's sarcoma cell line SLK was transfected by liposomes. After miR-181b-5p mimic or inhibitor was transfected,SLK cell proliferation was assessed by MTT assay. Cell cycle of each group was determined by flow cytometry after 48 hours after transfection. After miR-181b-5p mimic or inhibitor was transfected, cell migration and invasion was determined by transwell assay followed by hematoxylin staining after 24 hours. Results miR-181b-5p mimic promoted cell proliferation,promoted transformation and accelerated the process of cell cycle. miR-181b-5p inhibitor inhibited cell proliferation, induced G0/G1 phase retardation, delayed the process of cell cycle. Transwell migration assay results showed that number of migrated cell in the group treated with miR-181b-5p mimics was 184±9, significantly higher than that of control group 151±11, suggesting that miR-181b-5p mimics significantly increased cell migration ability(P<0.05). Transwell invasion assay showed that cell number of miR-181b-5p mimics group 48±5 significantly increased compared to negative control group 35±6 suggesting increased invasiveness(P<0.05). Conclusion miR-181b-5p may positively regulate the proliferation, invasion and migration of the SLK cell. miR-181b-5p may be a potential treatment taget for Kaposi's sarcoma.
keywords:miR-181b-5p  Kaposi's sarcoma  Cell cycle  Migration  Invasion
查看全文  查看/发表评论  下载PDF阅读器

京公网安备 11010502037822号