砖茶型氟铝联合应用对大鼠牙齿和骨代谢生化指标的影响
投稿时间:2014-12-13  修订日期:2014-12-29  点此下载全文
引用本文:孙中蕾,白静,陈瑶,刘庆斌.砖茶型氟铝联合应用对大鼠牙齿和骨代谢生化指标的影响[J].医学研究杂志,2015,44(11):111-114
DOI: 10.11969/j.issn.1673-548X.2015.11.031
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作者单位
孙中蕾 010110 呼和浩特, 内蒙古医科大学病理生理学教研室 
白静 010110 呼和浩特, 内蒙古医科大学病理生理学教研室 
陈瑶 010110 呼和浩特, 内蒙古医科大学病理生理学教研室 
刘庆斌 021000 呼伦贝尔市地方病防治研究所 
中文摘要:目的 观察砖茶型氟铝联合中毒对大鼠牙齿和骨代谢相关血清学指标的影响,以定量评估氟和铝对牙齿和骨代谢的损伤作用。方法 5周龄SPF级Wistar大鼠70只,随机分为7组,正常对照组,氟组,铝组,氟铝组,高剂量高氟高铝砖茶组,低剂量高氟高铝砖茶组,低氟低铝砖茶组。定期观察大鼠牙齿状态,使用酶联免疫吸附实验检测大鼠血清PTH、OT、CT水平,并且进行血清Ca、P、ALP的检测。结果 氟组、氟铝组、高剂量高氟高铝砖茶组均出现不同程度的氟斑牙;铝组除个别出现牙釉质光泽度消失外未见氟斑牙表现;低氟低铝砖茶组仅出现一例轻度氟斑牙。氟组、氟铝组、高剂量高氟高铝砖茶组的血清PTH、OT都高于对照组(P<0.01),且氟组最高;而铝组PTH,OT,ALP均较对照组降低(P<0.01);氟铝组和所有砖茶组的血清Ca水平都低于对照组(P<0.01),且低剂量高氟高铝砖茶组最低;氟组,氟铝组和高剂量高氟高铝砖茶组血清CT均比对照组低(P<0.01),以氟铝组最低,但铝组则呈显著性升高(P<0.01)。结论 本次研究的剂量条件下单氟对牙齿的损伤作用较明显,氟铝联合作用时表现为拮抗作用,且OT、PTH、CT和ALP能间接的反应骨骼的损伤情况。
中文关键词:砖茶  氟铝联合  牙齿  骨代谢
 
Nocuous Effects of Fluoride and Aluminum in the Brick Tea on the Teeth and Bone Metabolism in Rats.
Abstract:Objective To quantitatively evaluate the nocuous effects of fluoride and aluminum in the brick tea on the teeth and bone metabolism in a rat model. Methods Five-weeks old Wistar rats with both gender were randomly devided into 7 groups(n=10 in each group):i.e., the normal control, fluoride, aluminum, fluorine plus aluminum, high-dose fluoride-aluminum brick tea, low-dose fluoride-aluminum brick tea, and low fluoride and low aluminum brick tea group. Fluoride and aluminum were chronically administrated via regular chaw for 12 months. Then teeth injury was observed and serum parathyroid hormone(PTH), osteocalcin(OT), calcitonin(CT), alkaline phosphatase(ALP), calcium, and phosphorus were measured by ELISA. Results The teeth of normal rats were brown in color, smooth and shiny. Different degrees of dental fluorosis were found in groups of fluoride, fluoride plus aluminum and high-dose fluoride-aluminum brick tea. No dental fluorosis was found in the aluminum group; only one mild dental fluorosis was found in the low fluoride and low aluminum brick tea group. Serum PTH and OT in groups of fluoride, fluoride plus aluminum and high-dose fluoride-aluminum brick tea group were significantly higher(P<0.01) than the control group, and the highest was in the fluoride group. Serum PTH, OT and ALP in the aluminum group was lower than the control group(P<0.01); serum calcium in the fluorine plus aluminum group and the brick tea group was lower than the control group(P<0.01); serum CT in the fluorine group, the fluorine and aluminum group and the high-dose fluoride-aluminum brick tea group was lower than the control group(P<0.01), and CT was the highest in the fluorine and aluminum group, but CT level was decreased in the aluminum group. Conclusion Fluoride is the most potent in inducing teeth injury; aluminum might have a antagonistic effect on fluoride-induced teeth injury. Serum biomarkers of bone metabolism also indicate bone injury, and OT, PTH, CT and ALP are relatively more sensitive in indicating bone injury induced by fluoride and aluminum which were enriched in the brick tea.
keywords:Brick tea  F-Al combined toxicosis  Tooth  Mechanism  Bone metabolism
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