| 贝母素乙对放射性肺损伤大鼠肺组织水通道蛋白的影响 |
| 投稿时间：2015-03-26 修订日期：2015-04-23 点此下载全文 |
| 引用本文：吉福志,何栋成,龚婕宁,赵晓峰,郭海.贝母素乙对放射性肺损伤大鼠肺组织水通道蛋白的影响[J].医学研究杂志,2015,44(12):41-44 |
| DOI：
10.11969/j.issn.1673-548X.2015.12.012 |
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| 基金项目:国家自然科学基金资助项目(81373603);江苏省博士后科研资助计划第2批基金资助项目(1102170C);南京医科大学科技发展基金资助项目(2012NJMU153) |
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| 中文摘要:目的研究贝母素乙对肺损伤大鼠水通道蛋白AQP-1、AQP-5的影响,阐明贝母素乙治疗肺损伤的机制。方法将大鼠采用数字法随机分为4组:正常组(蒸馏水1ml/100g体重)10只,模型组(蒸馏水1ml/100g体重)12只、地塞米松组(等容积地塞米松蒸馏水溶液)12只、贝母素乙组(等容积贝母乙素蒸馏水溶液)12只,每组雌雄各半。6MV高能X线直线加速器15Gy/(3F·3W)照射,建立大鼠全肺放射性肺损伤模型。肺组织行HE染色,观察肺泡炎症程度。采用放射免疫法检测放射性肺损伤大鼠肺组织中AQP-1、AQP-5含量,荧光定量RT-PCR分析AQP-1、AQP-5 mRNA表达。结果贝母素乙可增高放射性肺损伤大鼠肺组织AQP-1、AQP-5水平,调节AQP-1、AQP-5 mRNA表达。结论贝母素乙具有调节AQP-1、AQP-5的作用,这可能是治疗放射性肺损伤作用机制的关键。 |
| 中文关键词:贝母素乙 水通道蛋白 放射性 肺损伤 |
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| Effect of Peiminine on Aquaporins of Lunl Injury in Rats. |
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| Abstract:Objective To study the effect of Peiminine on AQP-1 and AQP-5 against radiation-induced lung injury in rats and clarify its mechanism of treatment. Methods A total of 46 SD healthy rats were randomly assigned into 4 groups,including the comparison group,the radiation group,the dexamethaane group and the Peiminine group.The model in rats was established by irradiating whole rats chest with the dose of 15 Gy/3F of 6 MV X-rays.The activities of AQP-1 and AQP-5 in lung tissues were examined by immunoradiometric.AQP-1, AQP-5 expressions in lung tissues were detected by immunohistochemistry and RT-PCR. Results The results in our study indicated that peiminine had beneficial effects to protect against radiation-induced lung injury in rats.The attenuation of pulmonary fibrosis by peiminine was associated with a increasing in the levels of AQP-1, AQP-5, regulation of AQP-1, AQP-5 mRNA expression in lung tissue. Conclusion Our results thus provide evidence that peiminine have therapeutic potential for the treatment pulmonary fibrosis. |
| keywords:Peiminine Aquaporin Radioaction Lung injury |
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