| 儿童先天性晶状体半脱位术后房水中细胞因子的连续观察和临床分析 |
| 投稿时间：2015-04-27 修订日期：2015-05-22 点此下载全文 |
| 引用本文：丁宁,宋旭东,陶靖.儿童先天性晶状体半脱位术后房水中细胞因子的连续观察和临床分析[J].医学研究杂志,2015,44(12):45-50 |
| DOI：
10.11969/j.issn.1673-548X.2015.12.013 |
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| 基金项目:吴阶平医学基金会临床科研专项基金资助项目(320.6750.1365) |
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| 中文摘要:目的分析儿童先天性晶状体半脱位术前与术后不同时间房水中多种细胞因子的表达情况,探讨术后早期细胞因子的表达变化规律以及相关的临床意义。方法收集先天性晶状体半脱位患儿的房水样本31眼进行定量分析。其中术前取房水样本31眼,术后1周取房水样本10眼,术后1个月取房水样本13眼,术后2个月取房水样本8眼。房水样本应用流式细胞术微球阵列法(cytometric bead array,CBA)检测IL-1β、IL-2、IL-4、IL-5、IL-6、IL-10、TNF-α、IFN-γ 8种细胞因子的表达情况。结果术后1周患儿房水中IL-4(151.26±20.77fg/ml)、IL-6(153089.74±76980.97fg/ml)的表达量较术前升高,差异有统计学意义(P<0.05)。术后1个月患儿房水中IL-2(344.89±84.81fg/ml)、IL-4(149.89±37.14fg/ml)、IL-6[200000.00(129045.55)fg/ml]、TNF-α(274.32±57.40fg/ml)的表达量较术前升高,差异有统计学意义(P<0.05)。术后2个月患儿房水中IL-2(347.68±105.65fg/ml)、IL-4(153.92±29.69fg/ml)、IL-5(135.46±34.90fg/ml)、IL-6(123661.66±87444.85fg/ml)、IL-10(176.79±25.35fg/ml)、TNF-α(314.30±41.46fg/ml)的表达量仍高于术前,差异均有统计学意义(P<0.05)。IL-1β、IFN-γ表达差异无统计学意义(P>0.05)。结论儿童先天性晶状体半脱位患者手术前后房水中细胞因子的表达量可发生明显变化,术后房水细胞因子的表达升高可能与患儿手术后炎性反应及并发症的发生和疾病的预后有关。IL-4、TNF-α可能是对术后炎性反应变化较敏感的细胞因子;IL-6可能是术后炎性反应强度的预测因子。儿童内眼手术后应用抗炎药物的时间应适当延长。 |
| 中文关键词:晶状体半脱位 房水 细胞因子 儿童 |
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| Postoperative Progression of Cytokines in Aqueous Humor and Clinical Analysis of Children with Congenital Lens Subluxation. |
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| Abstract:Objective To investigate concentration of various cytokines in the aqueous humor of children with congenital lens subluxation and prospectively analyses the changes and clinical significance in the immediate postoperative period. Methods The staging operation was performed in 31 eyes of children with congenital lens subluxation and aqueous samples were collected at baseline (31 eyes), 1 week (10 eyes), 1 month (13 eyes) and 2 months (8 eyes) after surgery. Each sample was measured for 8 cytokines (IL-1β,IL-2,IL-4,IL-5,IL-6,IL-10,TNF-α,IFN-γ) using cytometric bead arrays (CBA), and we conducted quantitative analysis on the data. Results Aqueous humor levels of IL-4(151.26±20.77fg/ml) and IL-6(153089.74±76980.97fg/ml) were elevated at 1 week after surgery from baseline (P<0.05). IL-2(344.89±84.81fg/ml), IL-4(149.89±37.14fg/ml), IL-6[200000.00(129045.55)fg/ml] and TNF-α(274.32±57.40fg/ml) concentrations continued to steadily increase over the month (P<0.05). We found statistically significant differences for IL-2(347.68±105.65fg/ml), IL-4(153.92±29.69fg/ml), IL-5(135.46±34.90fg/ml), IL-6(123661.66±87444.85fg/ml)、IL-10(176.79±25.35fg/ml) and TNF-α (314.30±41.46fg/ml) between baseline and 2 months after surgery (P<0.05). We observed no significant differences concerning IL-1β and IFN-γ concentrations (P>0.05). Conclusion Great changes have taken place in cytokine concentrations in aqueous humor of children with congenital lens subluxation before and after surgery. Increased postoperative expression of cytokines in aqueous humor may contribute to the pathogenesis of inflammation and complications, and might be related to the prognosis of the disease. IL-4 and TNF-α are probably more sensitive to changes of the postoperative inflammatory response. IL-6 may be a predictor of inflammatory intensity after surgery. Anti-inflammatory drugs should be used appropriately extended in children after eye operation. |
| keywords:Lens subluxation Aqueous humor Cytokine Children |
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