| 肝癌组织hSulf-1基因表达与其甲基化状态的关系 |
| 投稿时间：2015-05-05 修订日期：2015-05-28 点此下载全文 |
| 引用本文：沈伟敏,刘凌,陆贝.肝癌组织hSulf-1基因表达与其甲基化状态的关系[J].医学研究杂志,2015,44(12):117-120 |
| DOI：
10.11969/j.issn.1673-548X.2015.12.032 |
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| 中文摘要:目的研究肝细胞肝癌组织中人硫酸酯酶-1(hSulf-1)基因甲基化的状态,其mRNA以及蛋白表达情况及与甲基化状态的相关性,探讨hSulf-1基因甲基化在肝癌发生发展中的作用。方法应用RT-PCR、甲基化特异性PCR及免疫印迹法,测定42例肝癌组织及其癌旁正常组织hSulf-1的mRNA、甲基化及蛋白表达水平,并分析hsulf-1基因表达水平及甲基化与肝癌临床特征的关系。结果在肝癌组织中,hSulf-1 mRNA和蛋白表达水平明显低于癌旁正常组织,而正常肝组织几乎检测不到。肝癌组织hSulf-1甲基化率为66.7%(28/42),而在癌旁正常组织中hSulf-1甲基化率为7.1%(3/42),二者之间差异有统计学意义(P<0.05),在hSulf-1 mRNA表达缺失或下调的31例肝癌组织中,其中有25例发生甲基化(χ2=8.45, P<0.05)。在肝癌组织中,甲基化组hSulf-1 mRNA及蛋白表达明显低于非甲基化组(分别为0.687±0.092 vs 2.324±0.123,P<0.01; 0.825±0.119 vs 2.212±0.178, P<0.01)。hSulf-1基因甲基化与患者肝硬化密切相关。结论肝细胞肝癌hSulf-1基因mRNA及蛋白表达明显下降、其甲基化程度明显增高,且hSulf-1基因的甲基化状态与mRNA及蛋白的表达情况具有某种联系,提示hSulf-1基因的甲基化状态可能与肝癌的发生、发展有关。 |
| 中文关键词:甲基化 肝细胞肝癌 hSulf-1 基因抑制 |
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| Influence of Methylation of hSulf-1 Gene on hSulf-1 mRNA and Protein Expression in Hepatocellular Cancer Tissues. |
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| Abstract:Objective To study the effect of hSulf-1 gene metheylation on mRNA and protein expression in colorectal cancer, and the role of hSulf-1 gene metheylation in pathogenesis of hepatocellular cancer. Methods mRNA, methylation and protein expression of hsulf-1 gene were detected by RT-PCR, methylation specific PCR and Western blott in 40 tumor and normal tissues of hepatocellular cancer. Results hSulf-1 mRNA and protein expression in tumor tissues were lower significantly than those in corresponding normal tissues of hepatocellular cancer. Methylation of hSulf-1 gene in the tumor tissues was detected at 66.7%, while the normal tissues had lower frequencies (7.1%). hSulf-1 mRNA and protein expression in methylation group of HCC tissues were lower than that in unmethylation group (0.687±0.092 vs 2.324±0.123, P<0.01; 0.825±0.119 vs 2.212±0.178, P<0.01 respectively).(4) Methylation of hSulf-1 gene in the tumor tissues was closely related to liver cirrhosis. Conclusion mRNA and protein expression levels of hSulf-1 is lower in the HCC tissue and low expression of hSulf-1 is associated with hypermethylation of hSulf-1 gene, which suggestes that hSulf-1 gene methylation plays a role in HCC carcinogenesis. |
| keywords:Methylation Hepatocellular cancer hSulf-1 Gene suppressor |
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