| 仙灵骨葆对去卵巢骨质疏松大鼠模型骨微结构的影响 |
| 投稿时间：2015-04-10 修订日期：2015-05-04 点此下载全文 |
| 引用本文：贺莉,李刚.仙灵骨葆对去卵巢骨质疏松大鼠模型骨微结构的影响[J].医学研究杂志,2015,44(12):160-163 |
| DOI：
10.11969/j.issn.1673-548X.2015.12.045 |
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| 中文摘要:目的观察仙灵骨葆对去卵巢骨质疏松大鼠模型骨微结构损伤的改善作用,并探讨其可能机制。方法30只雌性SD大鼠随机分为3组,正常组:卵巢假切+安慰剂,模型组:卵巢切除+安慰剂,治疗组:卵巢切除+仙灵骨葆。治疗组每天仙灵骨葆悬浊液灌胃,正常组和模型组按照相同剂量给予生理盐水灌胃,干预12周。实验结束后采集血清标本和骨骼标本做生化检测和骨微结构形态学分析。结果去卵巢大鼠雌二醇水平显著降低,血钙水平降低,碱性磷酸酶和骨钙素升高(P<0.05),仙灵骨葆能够改善上述现象;仙灵骨葆能够显著改善OVX大鼠松质骨丢失,显著增加BMD、Tb.Th、Tb.N、Tb.Sp和BV(P<0.05);治疗组皮质骨与正常组相比,Cr.Th、BV和BV/TV显著降低,PO则显著升高(P<0.05),但较模型组有所改善。结论仙灵骨葆具有延缓去卵巢大鼠发生骨密度下降、骨量减少及骨微结构破坏的作用,其机制可能与调控成骨细胞和破骨细胞活性及功能相关。 |
| 中文关键词:骨质疏松症 卵巢切除术 骨微结构 仙灵骨葆 |
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| Effect of Xianlinggubao on Bone Microstructure of OVX Rats. |
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| Abstract:Objective To observe the effect of Xianlinggubao on bone microstructure damage of ovary osteoporosis rat model, and discuss the possible mechanism. Methods Thirty female SD rats were randomly divided into 3 groups:normal group(sham plus placebo), model group(OVX plus placebo), treatment group(OVX plus Xianlinggubao). Treatment group was gavaged with Xianlinggubao suspension every day for 12 weeks, in accordance, normal group and model group were given same dose saline. In the end of experiment, serum samples and bone specimen were collected for biochemical detection and bone microstructure morphology analysis. Results The blood estradiol and calcium levels of OVX rats were significantly reduced, ALP and osteocalcin significantly increased (P<0.05), but xianlinggubao was able to improve the above symptoms. Xianlinggubao could improve the cancellous bone loss of OVX rats, significantly increased BMD, Tb.Th, Tb.N, Tb.Sp and BV (P<0.05). Compared with normal group, the cortical bone of treatment group, the index of Cr,Th, BV and BV/TV were significantly reduced, PO was significantly increased (P<0.05), but improved to model group. Conclusion Xianlinggubao could delay the bone mass lose and bone microstructure damage of OVX ruats. The mechanism may be related to regulation of osteoblast and osteoclast activity and function. |
| keywords:Osteoporosis OVX Bone microstructure Xianlinggubao |
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