| 敲除TSPAN1抑制人结肠癌细胞的生长和侵袭 |
| 投稿时间：2015-06-01 修订日期：2015-07-07 点此下载全文 |
| 引用本文：邢娜娜,张慧慧,谢佳琪,张泽延,杜润蕾,张晓东.敲除TSPAN1抑制人结肠癌细胞的生长和侵袭[J].医学研究杂志,2016,45(1):29-33 |
| DOI：
10.11969/j.issn.1673-548X.2016.01.008 |
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| 基金项目:国家自然科学基金资助项目(81270306,30971499) |
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| 中文摘要:目的 TSPAN1能够影响人结直肠癌细胞的增殖、扩散和侵袭。方法 用腺病毒同源重组的方法建立TSPAN1缺失的人结直肠癌HCT116细胞系,CCK-8、克隆形成、划痕和Transwell实验分别检测缺失TSPAN1的细胞生长速率、成瘤、迁移和侵袭能力。裸鼠成瘤实验检测TSPAN1对肿瘤扩散的影响。双荧光素酶实验验证TSPAN1对雌激素受体和TGF-β受体活性的影响。结果 缺失TSPAN1的HCT116细胞的生长速率、成瘤、迁移和侵袭能力显著降低。在裸鼠中分别注射野生型和缺失TSPAN1的HCT116细胞,后者肿瘤扩散变慢,并且裸鼠的成活率提高。过表达TSPAN1能够激活雌激素受体和TGF-β受体。结论 TSPAN1可能通过雌激素受体和TGF-β受体通路来调节细胞增殖和侵袭过程,并且为治疗结直肠癌提供了潜在的新靶标。 |
| 中文关键词:TSPAN1 结直肠癌 迁移 侵袭 TGF-β |
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| Knockout of TSPAN1 Inhibits Tumor Growth and Metastasis of Human Colon Cancer |
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| Abstract:Objective TSPAN1 affects human colon tumor cells on proliferation, migration and invasion. Methods We established a TSPAN1-deficient HCT116 cell line by adenovirus homologous recombination. Then we used CCK-8, colony formation, wound healing and Transwell assays to detect the rate of cell proliferation and the ability of tumor formation, migration and invasion of TSPAN1-deficient cells. With nude mouse tumorigenicity assay, we also tested the effect of TSPAN1 on the spread of tumor. Dual luciferase experiment validated that TSPAN1 effected the activity of estrogen receptor and TGF-beta receptors. Results The rate of cell proliferation and the ability of tumor formation, migration and invasion were significantly reduced in TSPAN1-deficient cells. We also injected HCT116 and TSPAN1-deficient HCT116 cells into athymic nude mice via the tail vein, and found a dramatic reduction of tumor cell migration. Mice injected with TSPAN1-deficient cells showed prolonged survival. Overexpression of TSPAN1 could active estrogen receptor and transforming growth factor-β receptor. Conclusion TSPAN1 might regulate cell migration and invasion through these pathways. Thus, we suggest that TSPAN1 may be a novel therapeutic target for colon cancer treatment. |
| keywords:TSPAN1 Colon cancer Migration Invasion TGF-β |
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