| 复方血栓通胶囊对糖尿病大鼠视网膜氧化应激损伤的保护作用 |
| 投稿时间：2015-06-19 修订日期：2015-06-24 点此下载全文 |
| 引用本文：邢玉微,邹俊杰,石勇铨,刘志民.复方血栓通胶囊对糖尿病大鼠视网膜氧化应激损伤的保护作用[J].医学研究杂志,2016,45(1):40-43 |
| DOI：
10.11969/j.issn.1673-548X.2016.01.010 |
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| 基金项目:国家重点基础研究发展计划(973)项目(2005CB523304) |
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| 中文摘要:目的 研究中药复方血栓通胶囊对糖尿病大鼠视网膜氧化应激损伤的保护作用。方法 SD雄性大鼠36只随机分为对照组(DM组,n=12)和造模组(n=24),造模组注射链脲佐菌素(STZ)60mg/kg,造模成功后随机分为糖尿病组(DM组,n=12),复方血栓通胶囊干预组[XST组,1g/(kg·d),n=12],3个月后处死大鼠,电镜观察视网膜组织结构,并且探查氧化应激指标超氧化物歧化酶(SOD)及丙二醛(MDA)含量,以实时定量检测生物活性因子血管内皮生长因子(VEGF)及诱导型一氧化氮合酶(iNOS)mRNA表达量。结果 相比于对照组,糖尿病组大鼠血糖升高,体重下降(P<0.05),电镜发现视网膜组织细胞出现氧化应激损伤表现,且氧化应激指标SOD下降, MDA升高(P<0.05),生物活性因子VEGF-mRNA及iNOS-mRNA明显升高(P<0.05);相比糖尿病组,复方血栓通胶囊干预组血糖、体重无明显变化(P>0.05),视网膜组织细胞损伤程度明显下降,氧化应激指标SOD、MDA及生物活性因子表达VEGF-mRNA、iNOS-mRNA均明显改善(P<0.05),但仍未达到对照组标准(P>0.05)。结论 高血糖通过氧化应激及增加生物活性因子VEGF及iNOS表达而损伤视网膜,而复方血栓通胶囊通过改善上述机制对高血糖造成的视网膜氧化应激损伤具有保护作用。 |
| 中文关键词:糖尿病视网膜病变 氧化应激 超氧化物歧化酶 丙二醛 诱导型一氧化氮合成酶 血管内皮生长因子 |
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| Protection of Fufang Xueshuantong Capsule Against Retinal Oxidative Damage of Diabetic Rats |
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| Abstract:Objective This study is to evaluate the potential protective effects of Fufang XuShuan Tong capsule against retinal oxidative damage in diabetes. Methods Adult male Sprague-Dawley rats were divided into 2group:control group(n=12)and model group(n=24).Rat diabetic model was induced by intraperitoneal injection of streptozotocin(60mg/kg body weight),and the model rats were randomly subdivided into diabetic group(DM,n=12),XST group[diabetic rats were treated with Fufang Xushuantong 1g/(kg·d),n=12]. After 3 months, all rats were executed. The retina tissue structure changes were observed by electron microscope and oxidative stress index superoxide dismutase (SOD) and malondialdehyde (MDA) levels was detected. Meanwhile, Biological active factors vascular endothelial cell growth factor (VEGF) mRNA and inducible nitric oxide synthase (iNOS)mRNA were also quantified in the retina by Realtime RT-PCR. Results Compared with the control group, in the DM group,blood glucose and body weight decreased (P<0.05), electron microscope showed that oxidative stress damage were found in retinal tissue, and oxidative stress index SOD level decreased and MDA level increased (P<0.05), the biological activity factor VEGF-mRNA and iNOS-mRNA levels increased significantly (P<0.05).Compared with DM group, in Fufang xushuan tong capsule intervention group,blood sugar level and weight showed no significant change (P>0.05), but retinal tissue damage degree decreased obviously. Oxidative stress index changes of SOD, MDA and biological active factor levels changes of VEGF-mRNA, iNOS-mRNA were significantly ameliorated (P<0.05), but still not reach the standard of the normal control group (P>0.05). Conclusion High blood glucose lead to retinal damage through oxidative stress and increasing biological activity factors VEGF and iNOS expression. Fufang Xushuantong capsule protects against high blood glucose-induced retinal oxidative damage through improving the above mechanism. |
| keywords:Diabetic retinopathy Oxidative stress Superoxide dismutase Malondialdehyde Inducibe nitric oxide synthase Vascular endothelial growth factor |
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