| 胚胎大鼠背根神经节神经元的原代培养 |
| 投稿时间：2015-06-10 修订日期：2015-06-29 点此下载全文 |
| 引用本文：孙青,梁晓春,张宏.胚胎大鼠背根神经节神经元的原代培养[J].医学研究杂志,2016,45(1):66-68,73 |
| DOI：
10.11969/j.issn.1673-548X.2016.01.017 |
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| 基金项目:北京市自然科学基金资助项目(7082077) |
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| 中文摘要:目的 原代培养胚胎大鼠背根神经节神经元(DRGn),并观察其生长特性。方法 取E15 SD胎鼠的背根神经节,用胰蛋白酶消化法分离成单细胞,通过密度梯度离心法和差速贴壁法进行分离纯化,在无血清培养基中培养,用抗神经元特异性烯醇化酶(NSE)抗体鉴定神经元。结果 纯化培养神经元生长状态良好,纯度可达93%左右。结论 该方法具有较好的可操作性和可重复性,用于DRGn的相关实验研究。 |
| 中文关键词:背根神经节神经元 原代培养 胚胎大鼠 |
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| Primary Culture of Embryonic Dorsal Root Ganglion Neurons in Rat |
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| Abstract:Objective To improve the methods for primary culture of embryonic dorsal root ganglion(DRG) neurons in rats. Methods DRGs were harvested from embryonic day 15 Sprague Dawley rats and dissociated in trypsin, purified by density gradient centrifugation and differential attachment technique, then cultured in neurobasal medium, and immunofluorescent method with neuronal specific enolase(NSE) antibody was used to identify them. Results DRG neurons survived and grew nicely in the medium, and the purified rate of DRG neurons could reach to around 93%. Conclusion It was a simple and reliable method to culture DRG neurons which can be used for the related researches. |
| keywords:Dorsal root ganglion neurons Primary culture Embryonic rats |
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