| 局部热激对大鼠睾丸生精细胞凋亡与增殖的影响 |
| 投稿时间：2015-07-09 修订日期：2015-07-21 点此下载全文 |
| 引用本文：张婷婷,何萌萌,郭健,李悦.局部热激对大鼠睾丸生精细胞凋亡与增殖的影响[J].医学研究杂志,2016,45(2):26-30 |
| DOI：
10.11969/j.issn.1673-548X.2016.02.008 |
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| 基金项目:国家自然科学基金资助项目(81273610) |
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| 中文摘要:目的 研究局部热激对大鼠睾丸生精细胞凋亡和增殖的动态变化及二者相关性的影响,以探索生精细胞凋亡与增殖的关系。方法 36只60天龄雄性Wistar大鼠随机分为对照组(22℃,20min)与热激组(43℃,20min),热激组按睾丸局部处理后1、2、3、4、5天分成5个亚组;采用HE染色分析各组大鼠曲细精管的形态变化;采用TUNEL法检测各组生精细胞凋亡情况;采用免疫组织化学方法(以下简称免疫组化)分析各组大鼠曲细精管Ki-67表达与分布的变化。结果 HE染色显示,热激后生精细胞逐渐脱落、缺失,至第4天最为严重,而第5天生精细胞有所恢复;TUNEL法检测显示,热激后各组的TUNEL凋亡信号显著增加(P<0.01),热激2天组达到高峰,热激3~5天组凋亡信号逐渐降低;免疫组化结果显示,热激后各组的Ki67表达显著下降(P<0.01),热激1天组很少见到Ki67表达,热激2~5天组Ki67表达逐渐增加;比较各组Ki67与TUNEL的比值,发现热激1~2天组二者比值小于1,热激3天组二者比值几乎等于1,热激4天组二者比值大于1。结论 睾丸局部热激可导致生精细胞凋亡,生精细胞增殖抑制,热激后早期凋亡过程胜于增殖,热激后期增殖强于凋亡过程,表明凋亡过程的可逆可能在于增殖活性增强。 |
| 中文关键词:热激 生精细胞 凋亡 增殖 |
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| Abstract:Objective To study the relationship between germ cell apoptosis and proliferation, thus to explore how the local exposure to heat stress affect the dynamic change and correlation between testicular germ cell apoptosis and proliferation was investigated. Methods Thirty-six male Wistar rats at the age of 60 days were randomly divided into control groups(22℃, 20min) and heat stress group(43℃, 20min), and the heat stress group was further divided into five sub-groups according to time 1 day, 2 days, 3 days, 4 days, 5 days after the testicular local heat stress. HE staining method was adopted to analyze morphological characteristics of seminiferous tubule. The germ cell apoptosis in all groups was examined by means of TUNEL. The immunohistochemical method was used to analyze the Ki67 distribution and expression in seminiferous tubule of all groups. Results The HE staining showed that germ cell gradually fell off and syarted missing after the testicular local heat stress, and the change was the most severe at the fourth day, but in the fifth day the germ cell had a little recovery. The TUNEL results showed that the TUNEL apoptosis signal increased significantly after testicular local heat stress(P<0.01) and it reached the peak at the second day. However, this TUNEL apoptosis signal in the 3-5d heat stress groups decreased gradually. The results of the immunohistochemistry showed that the Ki67 expression decreased significantly after the testicular local heat stress(P<0.01). The Ki67 expression in the 1d heat stress group was rarely seen, and the Ki67 expression in the 2-5d heat stress groups increased gradually. Compared to the ratio of the Ki67 and TUNEL among groups, it is found that the ratio in the 1-2d heat stress groups was less than 1. The ratio was almost equal to 1 in the 3d heat stress group. the ratio was greater than 1 in the 4d heat stress groups. Conclusion The testicular local exposure to heat stress increased testicular germ cell apoptosis and decreased testicular germ cell proliferation. The apoptosis process was superior to proliferation in the early phase after heat stress, but the proliferation was superior to apoptosis process in the late phase after heat stress, which indicated that the reversibility of apoptosis process could be dependent on stronger proliferation. |
| keywords:Heat stress Germ cell Apoptosis Proliferation |
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