| 粪菌移植在小鼠实验性结肠炎中的疗效研究 |
| 投稿时间：2015-06-10 修订日期：2015-07-21 点此下载全文 |
| 引用本文：姬盼盼,周中银,李櫆,操寄望,罗和生.粪菌移植在小鼠实验性结肠炎中的疗效研究[J].医学研究杂志,2016,45(2):54-58 |
| DOI：
10.11969/j.issn.1673-548X.2016.02.015 |
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| 基金项目:湖北省科技厅基金资助项目(2013BKB013) |
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| 中文摘要:目的 观察粪菌移植(fecal microbiota transplantation,FMT)对葡聚糖硫酸钠(dextran sulfate sodium,DSS)诱导的小鼠结肠炎的影响,探讨FMT对结肠炎的治疗作用和可能机制。方法 将小鼠分为4组:正常对照组、DSS组、美沙拉嗪(又叫5-氨基水杨酸,5-aminosalicylic acid,5-ASA)组、FMT组。除正常对照组外,其余3组小鼠连续饮用3%DSS水7天,建立急性溃疡性结肠炎(ulcerative colitis,UC)模型,同时DSS组、5-ASA组及FMT组于实验第1、3、5、7天分别给予0.5%羧甲基纤维素钠(Carboxymethylcellulose sodium,CMC-Na)、5-ASA及粪菌液灌肠。每天观察各组疾病活动指数(disease activity index,DAI),于实验第8天处死小鼠,测量结肠长度,检测结肠组织中中性细胞髓过氧化物酶(myeloperoxidase,MPO)活力,肿瘤坏死因子-α(tumor necrosis factor-alpha,TNF-α)、白介素1β(interleukin-1 beta,IL-1β)、白介素10(interleukin-10,IL-10)的含量。结果 与模型组相比,FMT可改善小鼠结肠组织炎症程度,降低MPO活力(P<0.05),减少组织TNF-α、IL-1β的含量(P<0.05),升高IL-10的含量(P<0.05)。结论 FMT对小鼠实验性结肠炎有治疗效果,可能通过重建肠道菌群,调节肠道T细胞免疫稳态来发挥治疗作用。 |
| 中文关键词:粪菌移植 肠道共生菌 溃疡性结肠炎 免疫稳态 |
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| Abstract:Objective To investigate the effect of fecal microbiota transplantation on acute inflammatory response in murine dextran sulfate sodium(DSS)-induced colitis, and its possible mechanism. Methods Mice were divided into four groups:normal control, DSS, 5-ASA and FMT. The mice in groups DSS, 5-ASA and FMT were orally administered with 3% DSS(w/v) solution for 7 days to induce colitis. At day 1, 3, 5 and 7 days, the mice in groups DSS, 5-ASA and FMT were respectively given 0.5% Carboxymethylcellulose sodium, 5-ASA suspension and fecal suspension by enema. Disease activity index(DAI) of every mouse was calculated every day. All mice were sacrificed at day 8 and the length of each colon was measured. The myeloperoxidase(MPO) activity and the content of TNF-α, IL-1β and IL-10 of inflamed colon of each group were measured. Results Compared with DSS group, FMT ameliorated inflammation severity of UC in mice accompanied by decreased MPO activity(P<0.05), reduced TNF-α, IL-1β levels(P<0.05) and increased IL-10 level(P<0.05)in colon tissue. Conclusion FMT showed a therapeutic effect on experimental colitis in mice. It was completed probably by reshaping intestinal flora, regulating intestinal T cell immunity homeostasis. |
| keywords:Fecal microbiota transplantation Intestinal symbiotic flora Ulcerative colitis Immunity homeostasis |
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