| 替格瑞洛对大鼠心肌缺血再灌注损伤的影响 |
| 投稿时间：2015-05-12 修订日期：2015-05-26 点此下载全文 |
| 引用本文：李康博,刘晓坤,张琦,韩全乐.替格瑞洛对大鼠心肌缺血再灌注损伤的影响[J].医学研究杂志,2016,45(2):79-82 |
| DOI：
10.11969/j.issn.1673-548X.2016.02.021 |
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| 基金项目:唐山市科技计划项目(13130258a) |
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| 中文摘要:目的 观察替格瑞洛对大鼠心肌缺血再灌注的影响,探讨其可能的作用机制。方法 成年健康雄性SD大鼠32只,采用随机区组法将其分为假手术组(SO组)、心肌缺血再灌注组(I/R组)、替格瑞洛组、替格瑞洛合并腺苷拮抗剂(CGS15943)组(替格瑞洛+CGS15943组)。制作大鼠缺血/再灌注损伤模型,SO组只穿线不结扎,替格瑞洛组术前连续强饲替格瑞洛,替格瑞洛+CGS15943组术前连续强饲替格瑞洛,术前腹腔内注射CGS15943。计算心肌梗死面积百分比,观察再灌注心律失常发生情况,检测血清中磷酸肌酸激酶(CK)和乳酸脱氢酶(LDH)水平及心肌组织超氧化物歧化酶(SOD)活力及丙二醛(MDA)含量。结果 替格瑞洛组大鼠心肌梗死面积小于I/R组(P<0.01);而替格瑞洛+CGS15943组则大于替格瑞洛(P<0.01);替格瑞洛组大鼠心律失常发生率、心律失常评分均低于I/R组(P均<0.05);而替格瑞洛+CGS15943组大鼠心律失常发生率、心律失常评分均高于替格瑞洛组(P均<0.05)。替格瑞洛组CK、LDH水平低于I/R组(P<0.05);而替格瑞洛+CGS15943组CK、LDH水平高于替格瑞洛组(P<0.05)。替格瑞洛组SOD活力高于I/R组(P<0.05),MDA含量低于I/R组(P<0.05);而替格瑞洛+CGS15943组SOD活力低于替格瑞洛组(P<0.05),MDA含量高于替格瑞洛组(P<0.05)。结论 替格瑞洛具有抗大鼠心肌缺血再灌注损伤保护作用,可能与其抑制腺苷代谢,从而减少氧自由基的产生有关。 |
| 中文关键词:替格瑞洛 腺苷 再灌注损伤 |
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| Abstract:Objective To observe the protective effects of pretreatment with ticagrelor on myocardial ischemia/reperfusion(I/R) iniury and related mechanism in rats. Methods Thirty-two healthy SD rats were randomly divided into sham operation(SO), myocardial ischemia and reperfusion(I/R), and ticagrelor, ticagrelor+an adenosine antagonist CGS15943. The infarction size and the incidence of ventricular arrhythmias were observed. The creatine phosphokinase(CK) and lactate dehydrogenese(LDH) of serum was detected at the end of the perfusion. The concentration of superoxide dismutase(SOD) and malonaldehyde(MDA) in the left ventricle was determined. Results Compared with I/R, ticagrelor significantly attenuated the myocardial infarct sizes and incidence of ventricular arrhythmias and arrhythmia score(P<0.05), and decreased the CK and LDH. The vitality of SOD in ticagrelor group was boosted(P<0.05), and the concentration of MDA in ticagrelor group was lower than that in I/R group(P<0.05), above effects of ticagrelor were partly abolished by co-treatment with CGS15943. Conclusion These data provide evidence for the first time that ticagrelor can provide remarkable protection against I/R injury in rat hearts via augmenting adenosine, which related to the anti-oxygen free radicals. |
| keywords:Ticagrelor Adenosine Ischemia/reperfusion injury |
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