哮喘患者血清中循环lncRNA差异性表达的初步筛查
投稿时间:2015-07-30  修订日期:2015-08-20  点此下载全文
引用本文:李博文,高卓,梁红艳,姜晓峰.哮喘患者血清中循环lncRNA差异性表达的初步筛查[J].医学研究杂志,2016,45(3):31-34
DOI: 10.11969/j.issn.1673-548X.2016.03.009
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作者单位E-mail
李博文 150001 哈尔滨医科大学附属第四医院检验科  
高卓 150001 哈尔滨医科大学附属第四医院检验科  
梁红艳 150001 哈尔滨医科大学附属第四医院检验科  
姜晓峰 150001 哈尔滨医科大学附属第四医院检验科 jiangxiaofeng12359@163.com 
基金项目:国家自然科学基金资助项目(81171657)
中文摘要:目的 对哮喘患者血清中差异性表达的lncRNA进行初步筛选,找到可以对哮喘诊断提供临床意义的潜在新型分子标志物.方法 收集哮喘患者血清50例,健康体检者血清32例,选择17种循环lncRNA,包括HULC、PRNCR1、TUSC7、PCGEM1、PCA3、TUC338、MEG3、UCA1、PTENP1、SNHG16、MALAT1、THRIL、NAET1、lnc-IL7R、GAS5、MIR155HG、TUG1.利用实时荧光定量PCR(RT-qPCR)技术检测lncRNA在血清中的差异性表达.结果 对17种lncRNA进行筛查表明,lncRNA PCGEM1在哮喘患者血清中表达水平显著低于体检者血清(P<0.01).另外,GAPDH作为内参,在筛选lncRNA的过程中表达稳定,其不受样本年龄、性别、发病情况的影响.结论 lncRNA PCGEM1在哮喘患者血清中显著低表达,可能成为临床诊断哮喘潜在的新型分子标志物.GAPDH可以作为本实验的循环lncRNA筛选的内参.
中文关键词:哮喘  循环长链非编码RNA  差异性表达  分子标志物
 
Preliminary Screening of Differential Expression of Circulating lncRNA in Serum of Asthmatic Patients.
Abstract:Objective LncRNAis selected for the differential expression of serum of asthmatic patients, in order to find a new molecular marker for diagnosis of asthma. Methods We collected serum of 50 cases of asthma patients and 32 cases of healthy volunteers.We choose 17 kinds of circulating lncRNA to test,inclduingHULC,PRNCR1,TUSC7,PCGEM1,PCA3,TUC338,MEG3,UCA1,PTENP1,SNHG16,MALAT1,THRIL,NAET1,Lnc-IL7R,GAS5,MIR155HG,TUG1.Real-time quantitativepolymerase chain reaction (RT-qPCR) was used to find differential expression of serum. Results The 17 kinds of lncRNA screening showed that the expression level of lncRNA PCGEM1 in serum of asthmatic patients was significantly lower than that in normal subjects. Also, GAPDH was used as internal reference, stably expressed in lncRNA screening process.It had no relation to age, gender and disease incidence. Conclusion The expression of lncRNA PCGEM1 in serum of asthmatic patients may be a novel molecular marker for clinical diagnosis of asthma. GAPDH can be used as the reference of circulating lncRNA screening.
keywords:Asthma  Circulating long non-coding RNA  Differential expression  Molecular marker
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