| 二烯丙基二硫对口腔鳞癌CAL-27细胞增殖及凋亡的研究 |
| 投稿时间：2015-07-22 修订日期：2015-08-26 点此下载全文 |
| 引用本文：刘鲁亮,李国林,顾博宇,迟惠熔,孙金环,郭福林.二烯丙基二硫对口腔鳞癌CAL-27细胞增殖及凋亡的研究[J].医学研究杂志,2016,45(3):115-118,130 |
| DOI：
10.11969/j.issn.1673-548X.2016.03.030 |
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| 基金项目:哈尔滨医科大学附属第一医院基金资助项目(2012B011) |
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| 中文摘要:目的 探讨二烯丙基二硫(diallyl disulfide, DADS)对口腔鳞癌CAL-27细胞的增殖抑制及促进凋亡的作用.方法 体外培养口腔鳞癌CAL-27细胞,采用不同浓度二烯丙基二硫对CAL-27细胞进行干预,并设立对照组,采用倒置显微镜观察细胞的形态学改变,MTT法检测细胞的增殖情况,Hoechst33342染色法检测细胞凋亡,用比色法检测caspase-3活性的改变.结果 倒置相差显微镜下观察对照组的细胞贴壁,形态呈多边形,生长活跃;实验组细胞形态改变,细胞脱壁,变小、变圆,细胞膜皱缩.MTT结果显示,随着浓度的增加,时间的延长,二烯丙基二硫对CAL-27细胞的生长及增殖有显著的抑制作用,并且各实验组相比及实验组与对照组相比,差异具有统计学意义(P<0.05).荧光显微镜下观察到经Hoechst33342染色的凋亡细胞.实验组caspase-3活性较正常对照组明显增高,差异有统计学意义(P<0.05).结论 二烯丙基二硫体外能抑制CAL-27的细胞增殖,呈一定的时间及剂量依赖性,并且诱导细胞凋亡,caspase-3可能参与了细胞凋亡的调控. |
| 中文关键词:口腔癌 CAL-27细胞 二烯丙基二硫 细胞增殖 细胞凋亡 |
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| Research on the Proliferation and Apoptosis of CAL-27 Induced by Diallyl Disulfide. |
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| Abstract:Objective To investigate the effects on CAL-27 cells proliferation and apoptosis induced by DADS. Methods CAL-27 cells were cultured in vitro.The oral squamous cell carcinoma CAL-27 cells cultured in medium were delt with different concentration of DADS at different time (24h, 48h, 72h). Morphological change of cells was observed by light microscope. The influence on the cell proliferation was investigated by MTT method. The cell apoptosis was detected by Hoechst33342 fluorescent staining. Moreover, the changes of caspase-3 activitity was evaluated by colorimeteric assay. Results Under light microscope, morphology of cells has not changed in the control, cell grow adherent, polygonal and actively. However, morphology of cells treated by DADS has changed. The cells became smaller and round, and shrank cells increased. Compared with the control, the cells of the experiment group were obviously inhibited. And there was significant difference of proliferation inhibition between them (P<0.05). A time and dose-dependent was detected by the MTT method. The significant cell apoptosis change could be observed with fluorescent microscopy. The level of caspase-3 activity were higher in the experiment group than control group (P<0.05). Conclusion DADS inhibits the growth and proliferation of CAL-27 cells and induce apoptosis. Furthermore, caspase-3 may be involved in the process of apoptosis. |
| keywords:Oral cancer CAL-27 cell Diallyl Disulfide Cell proliferation Cell apoptosis |
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