1型糖尿病小鼠肝脏线粒体电子传递链功能的研究
投稿时间:2015-09-22  修订日期:2015-10-09  点此下载全文
引用本文:于雪颖,米日阿依·阿里木江,殷峻.1型糖尿病小鼠肝脏线粒体电子传递链功能的研究[J].医学研究杂志,2016,45(4):22-25,28
DOI: 10.11969/j.issn.1673-548X.2016.04.007
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于雪颖 200233 上海交通大学附属第六人民医院内分泌代谢科、上海市糖尿病研究所、上海市糖尿病重点试验室、上海市糖尿病临床医学中心、上海市代谢病临床医学中心  
米日阿依·阿里木江 200233 上海交通大学附属第六人民医院内分泌代谢科、上海市糖尿病研究所、上海市糖尿病重点试验室、上海市糖尿病临床医学中心、上海市代谢病临床医学中心  
殷峻 200233 上海交通大学附属第六人民医院内分泌代谢科、上海市糖尿病研究所、上海市糖尿病重点试验室、上海市糖尿病临床医学中心、上海市代谢病临床医学中心 yinjun@sjtu.edu.cn 
基金项目:国家自然科学基金资助项目(31171128)
中文摘要:目的 探讨1型糖尿病小鼠肝脏线粒体功能的变化。方法 将10只6周龄C57BL/6J雄性小鼠随机分为两组:①腹腔注射链脲霉素造成1型糖尿病小鼠模型;②正常对照。实验期间监测血糖、体重等指标;造模结束后,用腹腔注射葡萄糖耐量实验(IPGTT)和胰岛素耐量实验(ITT)评估糖代谢,提取肝脏线粒体做氧化磷酸化测定,同时检测线粒体膜极性、钙离子蓄积能力、过氧化氢产生、柠檬酸合酶活性;检测血清和肝组织活性氧、总超氧化物歧化酶、丙二醛和总抗氧化能力等。结果 与对照小鼠相比,1型糖尿病小鼠在高血糖的同时胰岛素敏感度亦显著上调,肝脏线粒体分别加入复合物Ⅰ、复合物Ⅱ和复合物Ⅳ的底物后耗氧率明显上升(P<0.05);肝脏活性氧(P<0.01)和丙二醛(P<0.05)含量明显上升,总抗氧化能力明显下降(P<0.05)。结论 1型糖尿病小鼠肝脏线粒体电子传递链氧化磷酸化的活性明显增强,可能导致氧化应激的发生。
中文关键词:1型糖尿病  肝脏  线粒体  氧化磷酸化
 
Function of Hepatic Mitochondrial Electron Transport Chain in Type 1 Diabetic Mice
Abstract:Objective To investigate the changes in hepatic mitochondrial function of type 1 diabetic mice. Methods Six-week-old C57BL/6J male mice were randomly divided into 2 groups, one of which was treated with streptozotoc into develop type 1 diabetes, and the other was used as control group. The blood glucose and body mass of the mice were monitored during the experiment. After the development of type 1 diabetes, glucose metabolism was assessed with intraperitoneal glucose tolerance test and insulin tolerance test.The liver mitochondria were extracted andexamed for oxidative phosphorylation, membrane potential, calcium retention capacity, hydrogen peroxide production andcitate synthaseactivity. Total superoxide dismutase, malondialdehyde and total antioxidant capacity of liver, serum reactive oxygen species (ROS) and liver ROS were measured. Results Compared with control group, the oxygen consumption rate of hepatic mitochondrial ComplexⅠ, ComplexⅡ and Complex Ⅳ in the type 1 diabeteic group enhanced greatly(P<0.05). The total antioxidant capacity of the liver increased significantly (P<0.05).The hepatic ROS of the type 1 diabetes mice was increased significantly(P<0.01). Conclusion Compared with the control group, the activity of the hepatic mitochondria enhanced significantly.
keywords:Type 1 diabetic mice  Liver  Mitochondria  Oxidative phosphorylation
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