| 高危型人乳头瘤病毒E6/E7mRNA检测在宫颈人乳头瘤病毒阳性人群中的分流价值 |
| 投稿时间：2015-10-12 修订日期：2015-11-06 点此下载全文 |
| 引用本文：陈海迎,郑小冬,郑建琼,廖婵婵,吕杰强.高危型人乳头瘤病毒E6/E7mRNA检测在宫颈人乳头瘤病毒阳性人群中的分流价值[J].医学研究杂志,2016,45(5):141-145 |
| DOI：
10.11969/j.issn.1673-548X.2016.05.035 |
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| 基金项目:温州市科技局科研项目(Y20140546) |
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| 中文摘要:目的 探讨高危型人乳头瘤病毒(human papillomavirus,HPV)E6/E7mRNA检测在宫颈人乳头瘤病毒DNA阳性人群分流管理中的临床应用价值。方法 2014年1月~2015年7月因宫颈疾病在温州医科大学第三临床学院妇科门诊就诊的宫颈HPV-DNA阳性患者共970例,随机选取336例,均行HPVE6/E7mRNA及宫颈细胞学(TCT)检测,并追踪其病理学结果 。分析HPVE6/E7mRNA拷贝数与病理分级及宫颈细胞学结果 的关系,预测其对CINⅡ+的诊断价值。结果 鳞癌(squamouscell cancer,SCC)组拷贝数最高,无上皮内病变或恶性病变(negative for intraepithelial ledion or malignancy,NILM)组拷贝数最低,宫颈癌组HPVE6/E7mRNA阳性率及拷贝数最高,宫颈炎性改变组阳性率及拷贝数最低。不同细胞学诊断级别间HPVE6/E7mRNA水平比较,细胞学异常组(包括atypical squamous cells of undetermined significance,ASCUS,low-grade squamous intraepithelial lesion LISL,high-grade squamous intraepithelial lesions HISL,SCC)的表达水平高于NILM组,差异有统计学意义(F=28.99,P<0.01),不同宫颈组织病变程度级别间HPVE6/E7mRNA水平比较,CINⅡ+(histological dysplasia or cancer)的表达水平高于CINⅡ-,差异有统计学意义(F=40.154,P<0.01)。Spearman等级相关提示宫颈细胞学诊断级别及宫颈病理级别同HPVE6/E7mRNA拷贝数呈正相关,相关系数分别为(r=0.408,P<0.01;r=0.699,P<0.01)。结论 随着宫颈病变程度的加重,HPVE6/E7mRNA拷贝数逐渐增加,对宫颈HPV-DNA阳性患者的分流有一定的意义,对宫颈癌有一定的筛查价值。 |
| 中文关键词:人乳头瘤病毒 E6/E7mRNA 宫颈上皮内瘤变 HPV-DNA 分流 |
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| Clinical Performance of Human Papillomavirus(HPV) mRNA after Detection of HPV DNA |
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| Abstract:Objective To detect the role of Human papillomavirus(HPV) mRNA testing in the cervical cancer screening program in the triage of women harboring HPV DNA. Motheds A total of 336 patients were admitted to the Department of Obstetrics and Genecology,Wenzhou Third Clinical Institute of Wenzhou Medical University,the People's Hospital of Wenzhou from January 2014 to July 2015 .If the samples in case of HPV DNA detection were given cytologic examination, HPV E6/E7mRNA testing and colposcopic biopsy by histopathology.According to different cytologic examination and different histological results,the correlation between the expression of HPV E6/E7 mRNA were analyzed. Results The transcripts of the squamous cell cancer group were highest,while the negative for intraepithelial ledion or malignancy group were the lowest. The transcripts and positive rate of cervical cancer group were highest, while the chronic cervical inflammation group were the lowest. The levels of HPV E6/E7 mRNA in abnormal cytologic result groups (ASC,LISL,HISL,SCC) were significantly higer than that in NILM group respectively(F=28.99 ,P<0.01). The levels of HPV E6/E7 mRNA in CINⅡ+ (histological dysplasia or cancer) were significantly higer than that in CINⅡ-group respectively(F=40.154 ,P<0.01). Spearman rank analysis presented that the correlation between HPV E6/E7 mRNA levels with severity of cervical cytologic lesions(r=0.408,P<0.05) and histologic lesions(r=0.699, P<0.01)。 Conclusion With the development of cervical lesion,the expression of HPV E6/E7mRNA were increasing.Testing of HPV E6/E7mRNA may be usefull for the triage of women harboring HPV-DNA. |
| keywords:Human papillomavirus E6/E7mRNA Cervical intraepithelial neoplasia HPV-DNA Triage |
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