| 褪黑素联合顺铂对人胃癌细胞增殖及相关凋亡蛋白表达的影响 |
| 投稿时间：2015-07-03 修订日期：2015-07-15 点此下载全文 |
| 引用本文：范梦迪,李为民,陈依娜,宋彩云,颜页,吴建胜,郑超.褪黑素联合顺铂对人胃癌细胞增殖及相关凋亡蛋白表达的影响[J].医学研究杂志,2016,45(6):37-42 |
| DOI：
10.11969/j.issn.1673-548X.2016.06.010 |
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| 基金项目:国家自然科学基金资助项目（81200630）；浙江省自然科学基金资助项目（LQ12H07001） |
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| 中文摘要:目的 探究褪黑素（MLT）联合顺铂（CDDP）对人胃癌AGS细胞的疗效及相关凋亡蛋白表达的影响。方法 将体外培养的人胃癌细胞AGS细胞株分为4组，即AGS对照组、AGS MLT组（2mmol/L）、AGS CDDP组（4μg/ml）和AGS MLT+CDDP组（2mmol/L MLT和4μg/ml CDDP）。采用CCK8试验检测各组细胞增殖的抑制情况，Annexin Ⅴ FITC/PI凋亡试剂盒检测各组细胞早晚期细胞凋亡情况，Western blot法检测各组细胞凋亡蛋白表达情况。结果 MLT+CDDP组细胞的生存率明显低于AGS对照组和AGS 单药组；流式细胞术检测细胞凋亡情况表明，正常对照组、单药组和联合组细胞的早期凋亡率依次增加；蛋白水平研究表明，相比单药组和正常组，联合组的Bcl-2、原形态caspase-3表达显著降低，Bax及活化态cleaved-caspase-3表达明显增高。结论 与单用顺铂相比，褪黑素联合顺铂能明显抑制胃癌AGS细胞增殖并加强促凋亡能力，这可能与联合药影响相关凋亡蛋白表达有关。 |
| 中文关键词:褪黑素 顺铂 胃肿瘤 凋亡 增殖 |
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| Effect of Melatonin Combined with Cisplatin on Proliferation and Expression of Apoptotic Proteins in Human Gastric Cancer Cells |
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| Abstract:Objective To investigate the effect of melatonin combined with cisplatin on treatment and expression of apoptotic proteins in human gastric cancer AGS cells. Methods Human gastric cancer AGS cell strains culture in vitro were divided into four groups, namely AGS control group, AGS MLT group(3mmol/L),AGS CDDP group(4μg/ml) and AGS MLT+CDDP group(2mmol/L MLT and 4μg/ml CDDP). CCK-8 assay was used to test cell proliferation of each group. Annexin Ⅴ FITC/PI Apoptosis assay was used to test early or late apoptotic cells. Western blot was used to test the protein level of apoptotic proteins. Results The cell viability of AGS cells in MLT+CDDP group was the lowest in the whole groups. Results of Flow cytometry showed that the proportion of early apoptosis was increased in turn in normal control group, single drug groups and combination group. Compared with control group and single drug groups, the expression percentage of Bcl-2 and procaspase-3 decreased significantly in the combination group. On the contrary, the expression percentage of Bax and cleaved-caspase-3 increased significantly. Conclusion Compared with the effect of single cisplatin, the combination of melatonin and cisplatin is proved to be much more effective in the inhibition of proliferation and the induction of the apoptosis of human gastric cancer AGS cell strains, and the results perhaps were correlated with that combination drug could effect on the expression of apoptotic proteins. |
| keywords:Melatonin Cisplatin Stomach neoplasms Apoptosis Cell proliferation |
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