| 全脑照射对大鼠海马区Notch 1信号通路的影响 |
| 投稿时间：2015-12-01 修订日期：2016-01-05 点此下载全文 |
| 引用本文：应婷,丁维军,朱敏,于长辉,贾海建,杨海华.全脑照射对大鼠海马区Notch 1信号通路的影响[J].医学研究杂志,2016,45(6):69-73 |
| DOI：
10.11969/j.issn.1673-548X.2016.06.018 |
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| 基金项目:浙江省自然科学基金资助项目（Y2110739） |
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| 中文摘要:目的 观察大鼠全脑照射后海马区Notch 1信号通路中Notch 1与下游因子Hes 1的表达变化。方法 单次10Gy全脑照射构建大鼠放射性脑损伤模型，于照射后1、3天、1、2周、1、2个月处死大鼠获取海马组织，采用real-time PCR和Western blot法检测海马组织Notch 1、Hes 1的表达变化，免疫组织化学染色法检测海马区新生神经细胞数。结果 相较对照组，大鼠全脑照射后Notch 1表达进行性下调；其下游因子Hes 1在照射后2周内表达无明显变化，而在照射1、2个月后表达显著下调（P<0.05）。在照射后1、2个月组中，海马区新生神经细胞数较对照组显著减少。结论 大鼠全脑照射后，海马区Notch 1信号通路活性显著下调，伴随着海马区神经再生显著抑制，提示Notch 1通路可能参与放射性脑损伤后神经再生的调控过程。 |
| 中文关键词:海马 Notch 1信号通路 电离辐射 神经再生 |
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| Influence of Whole Brain Irradiation on Notch 1 Signaling Pathway in Rat Hippocampus |
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| Abstract:Objective To investigate whether whole brain irradiation could influence Notch 1 signaling pathway in rat hippocampus. Methods Female SD adult rats were suffered by a single dose of 10Gy X-ray whole brain radiation. Then, rats were sacrificed at 1 day, 3 days, 1 week, 2 weeks, 1 month and 2 months post radiation and hippocampuses were isolated. Real-time PCR and Western blot were performed to detect the expression change of Notch 1 signaling pathway. Immunohistochemistry staining was administrated to detect Newborn neurons in hippocampus by using doublecortin(DCX) as the marker. Results Compared to normal control group, the expression of Notch1 showed a tendency of progressive decrease at 1 day, 3 days, 1 week, 2 weeks, 1 month and 2 months after whole brain irradiation at hippocampus. Hes 1, a downstream target of Notch 1 signaling, stayed stable in 2 weeks post irradiation, and resulted in a significant reduction in expression at 1 month and 2 months post whole brain irradiation(P<0.05). Compared to normal control group, the number of newborn neurons was significantly reduced at 1 and 2 months after whole brain irradiation. Conclusion The expression of Notch 1 signaling was significantly downregulated post whole brain irradiation, accompanied by reduction of neurogenesis, which should be involved in the neurogenesis after whole brain irradiation in adult hippocampus. |
| keywords:Hippocampus Notch signaling pathway Ionizing radiation Neurogenesis |
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