| 大蒜素对高糖诱导人肾小管上皮细胞转分化的影响 |
| 投稿时间：2015-10-22 修订日期：2015-12-07 点此下载全文 |
| 引用本文：黄虹,江缨,袁放,郑和昕,阮园,吴天凤.大蒜素对高糖诱导人肾小管上皮细胞转分化的影响[J].医学研究杂志,2016,45(6):74-78,82 |
| DOI：
10.11969/j.issn.1673-548X.2016.06.019 |
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| 基金项目:浙江省中医药科学研究基金资助项目（2011ZA008），浙江省自然科学基金资助项目（Y2110918） |
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| 中文摘要:目的 探讨大蒜素对高糖诱导的人肾小管上皮细胞（HK-2）转分化的影响。方法 复苏并传代培养HK-2细胞，分为正常糖对照组（NG，5.5mmol/L）、高糖组（HG，25mmol/L）、高糖+不同剂量的大蒜素干预组（HG+2.5、5、10、20μg/ml Allicn）、高糖+JAK2抑制剂AG490组（HG+10μmol/L AG490）。倒置显微镜下观察高糖对细胞形态的影响，细胞免疫荧光检测α平滑肌肌动蛋白（α-SMA）、E钙黏蛋白（E-cadherin）、Ⅰ型胶原蛋白（collagen Ⅰ）的表达变化，荧光定量PCR检测 TGF-β1 mRNA的表达变化，Western blot法检测TGF-β1、p-STAT3、STAT3蛋白的表达变化。结果 与正常对照组相比，高糖刺激后的HK-2细胞的形态发生明显的长梭形改变，上皮细胞标志物E-cadherin表达明显减少，而间充质细胞标志物ɑ-SMA及collagen Ⅰ的表达明显升高（P<0.01）；TGF-β1及p-STAT3的表达明显增高（P<0.05）；而加入大蒜素或JAK2抑制剂AG490后，均能不同程度抑制高糖诱导的HK-2细胞转分化，而且大蒜素可明显抑制TGF-β1及p-STAT3的表达，以20μg/ml尤为显著（P<0.05）。结论 大蒜素可能通过抑制JAK2/STAT3信号通路活化，从而抑制高糖诱导的HK-2细胞转分化。 |
| 中文关键词:大蒜素 高糖 肾小管上皮细胞转分化 TGF-β1 JAK2/STAT3信号通路 > |
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| Effects of Allicin on Human Tubular Epithelial-myofibroblast Transdifferentiation Induced by High Glucose |
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| Abstract:Objective To investigate the effect of Allicin on high glucose-induced human renal proximal tubular epithelial myofibroblast transdifferentiation(TEMT). Methods Cultured HK-2 cells were divided into 7 groups:normal glucose (NG, 5.5mmol/L), high glucose (HG, 25mmol/L), high glucose with Allicin treatment (HG+2.5,5,10,20μg/ml Allicin) and high glucose with the JAK2 inhibitor AG490 group (HG+10μmol/L AG490). The cells were cultured for 48h. Inverted microscopy was used to observe the morphological changes in high glucose-induced HK-2 cells.Expression of α-smooth muscle actin (α-SMA), E-cadherin, collagen Ⅰ were detected by immunofluorescence assay, the expression of TGF-β1 mRNA were measured by real-time PCR, and that of TGF-β1、p-STAT3、STAT3 were measured by Western blot. Results After incubated with high glucose, HK-2 cells turned into long spindle-shape compared with NG group. The expression of epithelial marker E-cadherin was significantly decreased, while those of Collagen Ⅰ and the mesenchymal markers α-SMA were significantly increased (P<0.01). The expression of TGF-β1 and p-STAT3 were significantly increased (P<0.05). The administration of Allicin or AG490 obviously inhibited EMT (especially at 20μg/ml, P<0.01) in HK-2 cells induced by high glucose, while Allicin markedly decreased the expression of TGF-β1 and p-STAT3 (especially at 20μg/ml, P<0.05). Conclusion Allicin may inhibit tubular epithelial-myofibroblast transdifferentiation via at least regulating JAK2/p-STAT3 signaling pathway. |
| keywords:Allicin High glucose Transdifferentiation of renal tubular epithelial cells TGF-β1 JAK2/STAT3 |
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