| 高糖活化小鼠小胶质细胞BV-2模型的建立 |
| 投稿时间：2015-11-11 修订日期：2015-12-07 点此下载全文 |
| 引用本文：景光婵,张孟仁.高糖活化小鼠小胶质细胞BV-2模型的建立[J].医学研究杂志,2016,45(7):40-43 |
| DOI：
10.11969/j.issn.1673-548X.2016.07.012 |
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| 基金项目:北京市中医药科技项目（JJ2014-50） |
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| 中文摘要:目的 观察高糖（Glu）对小鼠神经小胶质细胞BV-2活化的影响。方法 将BV-2细胞分成：正常对照组（25mmol/L Glu），脂多糖组（1μg/ml LPS），高糖组（75mmol/L Glu），培养24h，倒置相差显微镜观察细胞形态，MTT检测细胞活力，激光扫描共聚焦显微技术、Western blot法及实时荧光定量PCR技术检测BV-2活化的标志物CD11b的表达。结果 高糖组、LPS组细胞活力与正常对照组相比差异均无统计学意义（P >0.05）；高糖组BV-2 CD11b蛋白含量较正常对照组显著升高（P<0.01），与LPS组类似（P >0.05）；高糖组BV-2 CD11b-mRNA值较正常对照组显著升高（P<0.01），且显著高于LPS组（P<0.01）。结论 75mmol/L高糖培养BV-2 24h，在不影响细胞活力的条件下，可显著上调BV-2活化标志物CD11b蛋白及mRNA表达，作用与LPS相当。 |
| 中文关键词:高糖|小胶质细胞|活化|脂多糖 |
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| Establishment of a High Glucose-induced Microglia Activation Model |
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| Abstract:Objective To observe the effect of high glucose on the activation of microglia BV-2 cells. Methods The BV-2 cells incubated in vitro were divided into three groups:control group(25mmol/L Glu), LPS group(1μg/ml),high glucose group(75mmol/L Glu), After being cultured for 24h under different conditions, the morphological changes of microglia BV-2 cells was observed by inverted phase contrast microscope, MTT colorimetric assay was adopted to measure BV-2 proliferation, confocal laser scanning microscopy, western blot and real-time fluorescence quantitative PCR were explored to determine the expression of surface markers CD11b of activated microglia. Results MTT colorimetric assay:After 24h, compared with control group, the OD values of high glucose group, LPS group and different NFC groups showed no significant differences(P >0.05).The expression of CD11b in the Glu group increased significantly compared with those of control group (P<0.01),which showed no statistical difference comparing with LPS positive group(P >0.05). The level of CD11b mRNA transcription in the Glu group were much higher than those of the Con group and LPS positive group. Conclusion After stimulation with high glucose (75mmol/L) for 24h, the transcription and expression of surface expression of activation marker CD1lb in microglia BV-2 cells were noticeably up-regulated, while the vitality of microglia BV-2 cells was not influenced. |
| keywords:High glucose|Microglia|Activation|LPS |
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