| Abstract:Objective To observe the effects of reduced glutathione (GSH) on oxidative stress and extracellular matrix of mouse glomerular mesangial cells in hyperglycemic milieu. Methods The cultured mouse glomerular mesangial cells were divided into 5 groups:normal glucose group (5mmol/L), high glucose group (30mmol/L), and GSH groups (high glucose with 1mmol/L, 5mmol/L, 10mmol/L GSH respectively). The cell culture supernatant were saved for detecting the concentrations of MDA, H2O2, NO, tSOD and TGF-β1, FN, LN at 72h after different treatments by ELISA assay. Results Compared with the normal glucose group, the concentrations of MDA, H2O2, NO and TGF-β1, FN, LN were increased and the concentration of tSOD was decreased in high glucose group (P<0.05). The concentrations of MDA, H2O2, FN and LN were reduced by treatment of different concentrations of GSH (P<0.05), and the concentrations of NO, TGF-β1 were inhibited by GSH 5 and 10mmol/L, and the concentration of tSOD was increased by 10mmol/L of GSH (P<0.05). Conclusion GSH could inhibit oxidative stress and extracellular matrix of mouse glomerular mesangial cells induced by hyperglycemic milieu, and may be helpful in the treatment of diabetic nephropathy. |
