| 葛根素对重度烧伤大鼠心肌细胞凋亡的影响及其机制研究 |
| 投稿时间：2015-01-02 修订日期：2016-01-11 点此下载全文 |
| 引用本文：童海东,戴如冰,陈骅.葛根素对重度烧伤大鼠心肌细胞凋亡的影响及其机制研究[J].医学研究杂志,2016,45(8):145-149 |
| DOI：
10.11969/j.issn.1673-548X.2016.08.038 |
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| 中文摘要:目的 观察葛根素（Puerarin）对重度烧伤大鼠心肌细胞凋亡的影响，并探讨其可能的作用机制。方法 取实验用大鼠120只随机分为正常对照组、模型组、葛根素（50、100、200mg/kg）组和地塞米松（5mg/kg）组；除正常对照组外，其余各组大鼠均采用脱毛后于92℃水浴18s的方法制备30% TBSA Ⅲ度烧伤模型大鼠；造模完成后，各组大鼠均立即腹腔注射乳酸林格液（40mg/kg）进行复苏；各治疗组在复苏同时腹腔注射给药进行治疗，正常对照组和模型组分别给予等体积生理盐水。24h后，通过末端标记法（Tunel）检测心肌细胞凋亡状况并计算凋亡指数（AI），通过免疫组织化学法（IHC）检测心肌组织中bcl-2和Bax蛋白表达并进行半定量分析、计算bcl-2/Bax表达比值，通过Western blot法检测心肌组织中caspase-3、NF-κB蛋白表达并进行半定量分析；测定心肌组织中超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）、髓过氧化物酶（MPO）活性和丙二醛（MDA）含量。结果 与模型组比较，葛根素各组心肌细胞凋亡数量呈不同程度减少，葛根素（100、200mg/kg）组AI显著降低（P<0.05，P<0.01），心肌组织中bcl-2表达显著上调、Bax表达显著下调，bcl-2/Bax表达比值显著升高（P<0.01），caspase-3蛋白和NF-κB蛋白表达量显著下调（P<0.05，P<0.01），心肌组织中SOD、GSH-Px活性显著升高且MDA含量显著降低（P<0.05，P<0.01），其中葛根素200mg/kg组MPO活性显著升高（P<0.01）。结论 葛根素具有抑制重度烧伤大鼠心肌细胞凋亡的作用，表现出对烧伤大鼠心肌组织较好的保护作用，作用机制可能与葛根素能够调节凋亡相关基因和蛋白表达以及降低氧化应激损伤有关。 |
| 中文关键词:葛根素 烧伤 心肌细胞 凋亡 机制 |
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| Effects and Mechanism of Puerarin on Myocardial Apoptosis in Severely Burned Rats |
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| Abstract:Objective To investigate the effects and mechanism of puerarin on myocardial apoptosis in severely burned rats. Methods One hundred and twenty experimental rats were randomly devided into six groups: normal control, model control group, puerarin 50, 100, 200mg/kg treated groups and dexamethasone 5mg/kg treated group. Except the rats in normal control group, the model rats with 30% TBSA (three degree) were made by bathing in 92℃ water for 18s after epilation. And then, the rats were given Ringer (40mg/kg) by intragastric administration to resuscitate. At the same time, the drugs were given by intragastric administration. Twenty-four hours later, the apoptosis of cardiomyocytes was observed by TUNEL staining, and the apoptosis index(AI) was calculated.The expression of Bax, bcl-2 were determined by IHC and they were Semi-quantitative analysised, and the ratio of bcl-2/Bax was calculated. The expression of caspase-3, NF-κB was determined by Western blot and it was Semi-quantitative analysised. The activity of SOD, GSH-Px, MPO and the content of MDA in myocardial tissue were measured. Results Compared with model control group, the apoptosis of cardiomyocytes in puerarin treated groups were significantly decreased, and the AI of puerarin(100, 200mg/kg) treated groups were significantly decreased(P<0.01). The expression of bcl-2 was significantly up-regulated and the Bax was significantly down-regulated, and the ratio of bcl-2/Bax was significantly increased(P<0.05, P<0.01). The expression of caspase-3, NF-κB in puerarin(100, 200mg/kg) treated groups were significantly decreased. The activity of SOD, GSH-Px in myocardial tissue of puerarin(100, 200mg/kg) treated groups were significantly increased and the content of MDA were significantly decreased(P<0.05, P<0.01), and the activity of MPO of puerarin 200mg/kg treated group was significantly increased(P<0.01). Conclusion Puerarin had inhibitive effects on myocardial apoptosis in severely burned rats, suggesting that puerarin had better protective effects on the myocardial tissue in severely burned rats.It perhaps related to its effects of regulating the expression of apoptosis-related genes and protein, and inhibiting oxidative stress. |
| keywords:Puerarin Burn Cardiomyocytes Apoptosis Mechanism |
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