| 大鼠大脑皮质星形胶质细胞α2A型肾上腺受体的表达研究 |
| 投稿时间：2016-03-01 修订日期：2016-03-30 点此下载全文 |
| 引用本文：罗雪妮,杨振宇,付豹,张宇,喻田.大鼠大脑皮质星形胶质细胞α2A型肾上腺受体的表达研究[J].医学研究杂志,2016,45(9):52-55 |
| DOI：
10.11969/j.issn.1673-548X.2016.09.014 |
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| 基金项目:国家自然科学基金资助项目（81460219） |
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| 中文摘要:目的 验证大鼠大脑皮质星形胶质细胞中的α2A肾上腺素能受体（α2A-AR）的表达情况，并与α2B肾上腺素能受体（α2B-AR）、α2C肾上腺素能受体（α2C-AR）相比较。方法 获取目的基因α2A-AR、α2B-AR、α2C-AR的RNA反转录，与内参基因β-actin一起进行实时荧光定量PCR。重复6组，所得数据进行统计学分析并运用2-△△Ct的方法进行计算。结果 大鼠大脑皮质星形胶质细胞中确实表达了α2A-AR，并且α2A-AR的相对表达量是α2B-AR的24.53倍，是α2C-AR的27.98倍。结论 α2A-AR可能为大鼠大脑皮质星形胶质细胞接受儿茶酚胺类神经递质调控的主要受体。 |
| 中文关键词:α2A肾上腺素能受体 星形胶质细胞 实时荧光定量PCR |
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| Investigation on the Expression of the α2A Adrenergic Receptor in the Cerebral Astrocytes of Rats |
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| Abstract:Objective To testify the expression of α2A adrenergic receptor(α2A-AR)in the cerebral astrocytes of rats, and compare the expression level of α2A adrenergic receptor with the α2B adrenergic receptor(α2B-AR) and α2C adrenergic receptor(α2C-AR). Methods Cerebral astrocytes were get from SD rats (aged 1-3 days). High purity of astrocytes were tested. We extracted and reversely transcribed the target RNA of α2A-AR,α2B-AR and α2C-AR from the astrocytes, then Real-time PCR was performed together with the house gene β-actin. The aborer proledures were repeated six times, and all data were used for statistical analysis and a method ofto caculate the results. Results α2A-AR were found markablely expressed in the cerebaral astrocytes of rats, which was 24.53 times to the expression of α2B-AR and 27.98 times to the expression of α2C-AR. Conclusion Based on the results, we infer that the α2A-AR is the main adrenergic receptor in the cerebaral astrocytes of rats that receiving catecholamine regulation. |
| keywords:α2A Adrenergic receptor Astrocytes Real-time PCR |
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