| MiR-148a调控胃癌细胞侵袭迁移的作用机制研究 |
| 投稿时间：2016-03-06 修订日期：2016-03-30 点此下载全文 |
| 引用本文：吴洁,缪林.MiR-148a调控胃癌细胞侵袭迁移的作用机制研究[J].医学研究杂志,2016,45(10):99-102 |
| DOI：
10.11969/j.issn.1673-548X.2016.10.026 |
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| 中文摘要:目的 研究miR-148a在胃癌侵袭迁移中的作用，为新肿瘤标志物的研究提供理论基础。方法 荧光实时定量PCR（RT-PCR）检测miR-148a在胃癌细胞系和正常胃黏膜细胞中的表达水平，HGC-27分别转染miR-148a control/miR-148a mimic和miR-148a control/miR-148a inhibitor后检测miR-148a表达水平的改变，Transwell法检测miR-148a对HGC-27细胞侵袭和迁移的影响，Western blot法检测miR-148a对胃癌细胞中c-myc蛋白表达的调控。结果 MiR-148a在胃癌细胞中的表达明显低于正常胃黏膜细胞；HGC-27转染miR-148a mimic后miR-148a表达升高，HGC-27转染miR-148a inhibitor后miR-148a表达下降；miR-148a能够显著抑制HGC-27细胞的侵袭和迁移；miR-148a能够明显下调c-myc蛋白的表达。结论 miR-148a通过c-myc来发挥抑制胃癌侵袭迁移的作用。 |
| 中文关键词:miR-148a 胃癌 侵袭迁移 |
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| Mechanism of Invasion and Metastasis in Gastric Cancer Regulated by miR-148a |
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| Abstract:Objective To study the role of miR-148a in regulation of invasion and metastasis in gastric cancer, to provide a theoretical basis for the study of novel tumor markers. Methods In this study, the mRNA expression levels of miR-148a were examined in GC cell lines and normal gastric mucosa cells line by quantitative reverse transcription-PCR. The functions of miR-148a in migration/invasion were determined by using transwell. The expression of miR-148a in the HGC-27 cell after tranfected with miR-148a mimic or miR-148a inhibitor was detected using qRT-PCR. The protein expression of c-myc was measured by western blot. Results The expression of miR-148a was downregulated in the GC cell lines (SGC-7901, MGC-803, HGC-27, MKN-45 and BGC-823) compared with normal gastric mucosa cells line (GES-1).After tranfected with miR-148a mimic, the expression of miR-148a increased in HGC-27 cell, but miR-148a inhibitor decreased the expression of miR-148a in HGC-27. HGC-27 infected with miR-148a inhibitors showed increased cell invasion and migration. We also observed that HGC-27 cell expressing miR-148a mimics exhibited reduced migration rates compared with the controls. Our results show that ectopic expression of miR-148a decreased c-myc in gastric cancer cell line. Conclusion miR-148a suppresses gastric cancer cell invasion and migration by inhibiting c-myc. |
| keywords:miR-148a Gastric cancer Invasion and metastasis |
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