| 两种脑出血动物模型的制备和比较研究 |
| 投稿时间：2016-05-19 修订日期：2016-05-26 点此下载全文 |
| 引用本文：魏纯纯,王培,缪朝玉.两种脑出血动物模型的制备和比较研究[J].医学研究杂志,2016,45(11):39-44 |
| DOI：
10.11969/j.issn.1673-548X.2016.11.011 |
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| 基金项目:国家自然科学基金资助项目（81373414，81130061，81422049，81473208）；国家“863”青年科学家项目（2015AA020943） |
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| 中文摘要:目的 本研究制备两种脑出血动物模型：脑缺血后使用tPA诱导的出血转化模型（MCAO-HT）及胶原酶注射诱导的脑出血模型（cICH），并比较两种出血模型的异同及可能的优缺点。方法 MCAO-HT模型：栓线经颈内动脉入颅，堵塞大脑中动脉，构建线栓法大脑中动脉堵塞模型，堵塞后5h静脉注射组织型纤溶酶原激活剂（tPA，10mg/kg）制备脑缺血后出血转化模型；cICH模型：利用脑立体定位技术，直接向纹状体注射0.05U胶原酶Ⅳ，诱导脑出血模型。结果 MCAO-HT模型小鼠较MCAO模型小鼠，24h患侧半脑血红蛋白含量显著升高，MCAO-HT模型小鼠血-脑脊液屏障（BBB）通透性显著高于MCAO模型小鼠；cICH模型小鼠，较假手术组小鼠，24h神经功能损伤、脑水肿、出血量和炎性水平都有显著升高。结论 MCAO-HT模型引起的出血为tPA诱导BBB通透性增加，导致的“渗血”，可能更适用于对血-脑脊液屏障及血管通透性的研究。而cICH模型引起的出血为胶原酶直接破坏小血管壁导致的“漏血”，可能更适用于出血诱导的水肿、炎性反应、细胞死亡和氧化应激的研究。 |
| 中文关键词:脑缺血 出血转化 脑出血 动物模型 |
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| Construction and Comparison of Two Animal Models of Cerebral Hemorrhage |
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| Abstract:Objective To construct two animal models of cerebral hemorrhage:tPA-induced cerebral ischemia hemorrhagic transformation (MCAO-HT) model and collagenase-induced ICH (cICH) model, and compare the similarities and differences of these two intracerebral hemorrhage models. Methods MCAO-HT model:We built suture MCAO model by blocking of the middle cerebral artery with suture, after five hours, we injected tissue plasminogen activator (tPA, 10mg/kg) intravenously to induce hemorrhagic transformation. cICH model:intracerebral hemorrhage model was induced by stereotaxicion of 0.05U collagenase Ⅳ into the striatum. Results In comparison with MCAO model mice, ipsilateral hemisphere hemoglobin and blood brain barrier (BBB) permeability in MCAO-HT model mice increased significantly at 24 hours after surgery. Compared with sham-operated mice, neurological damage, cerebral edema, hemorrhage inflammation levels had increased significantly in cICH model mice at 24 hours after surgery. Conclusion MCAO-HT model induced bleeding functioned by tPA-induced increasing of BBB permeability, which might be better for blood-brain barrier and vascular permeability study. Bleeding of cICH model caused by collagenase induced small blood vessels rupture, which suggested to be a better method to do the hemorrhage-induced edema, inflammation, cell death and oxidative stimulated research. |
| keywords:Cerebral ischemia Hemorrhagic transformation Intracerebral hemorrhage Animal model |
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