| 高脂饮食大鼠脂肪肝血清抵抗素的变化及多烯磷脂酰胆碱的干预作用 |
| 投稿时间：2016-02-24 修订日期：2016-04-17 点此下载全文 |
| 引用本文：车财妍,李红山.高脂饮食大鼠脂肪肝血清抵抗素的变化及多烯磷脂酰胆碱的干预作用[J].医学研究杂志,2016,45(11):121-123 |
| DOI：
10.11969/j.issn.1673-548X.2016.11.031 |
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| 基金项目:宁波市自然科学基金资助项目（2012A610243） |
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| 中文摘要:目的 研究血清抵抗素在实验性脂肪肝中的变化及多烯磷脂酰胆碱的干预作用。方法 采用单纯高脂饮食诱导的大鼠脂肪肝模型，随机分正常组、模型组和多烯磷脂酰胆碱组，造模12周，其多烯磷脂酰胆碱组在第8周起予以多烯磷脂酰胆碱灌胃干预5周。观察项目：肝组织HE染色，观察各组肝脂肪变性程度变化；肝组织甘油三酯（triglyceride，TG）、游离脂肪酸（free fatty acid，FFA）、血清抵抗素含量；肝组织TG、FFA、血清抵抗素含量间的相关性分析。结果 模型组肝组织出现严重的脂肪变性，肝组织TG、FFA含量均显著升高（t值分别为14.85和19.61，P<0.01）；血清抵抗素含量亦显著升高（t=12.94，P<0.01）；多烯磷脂酰胆碱组的TG、FFA含量显著低于模型组（P<0.01），血清抵抗素含量亦显著低于模型组（P<0.01）。肝脏TG、FFA含量与血清抵抗素含量之间均呈正相关。结论 实验性脂肪肝大鼠的血清抵抗素水平显著升高，在脂肪肝病理机制中有重要意义。多烯磷脂酰胆碱可显著降低脂肪肝大鼠的血清抵抗素水平，这可能是其防治脂肪肝作用的重要药理环节。 |
| 中文关键词:血清抵抗素 脂肪肝 多烯磷脂酰胆碱 |
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| Changes of Serum Resistin on Fatty Liver Disease in Rats Induced with High-fat Diet and the Polyene Phosphatidylcholine Intervention |
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| Abstract:Objective To investigate the changes of the serum resistin in experimental fatty live rand the effect of Polyene phosphatidylcholine on fatty liver disease. Methods Fatty liver in SD male rats were induced with high-fat diet for 12 weeks. Rats were divided into normal, model and Polyene phosphatidylcholine group and administrated with Polyene phosphatidylcholine for five weeks at the beginning of the eight week. The observing items including:Hepatic steatosis (HE staining); The serum resistin, hepatic triglyceride (TG) and free fatty acid (FFA); The correlation analysis among serum resistin, hepatic TG and FFA. Results Serious hepatic steatosis, increased hepatic TG and FFA were all observed in the model group(P<0.01), as well as the increased serum resistin content (P<0.01). Compared with model group, the triglyceride and free fatty acid content in liver tissue of polyene phosphatidylcholine group was reduced significantly(P<0.01), and the serum resistin content in liver tissue of polyene phosphatidylcholine group was reduced significantly too(P<0.01). Significant positive correlation was observed between hepatic TG, FFA and serum serum resistin. Conclusion Serum resistin significantly increased in experimental fatty liver in rats which probably plays very important roles in pathological mechanisms of fatty liver disease. Polyene phosphatidylcholine can decreased the serum resistin markedly, which probably is the key mechanism of its prevention and treatment effect on fatty liver disease. |
| keywords:Serum fesistin Experimental fatty liver Polyene phosphatidylcholine |
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