| 乳康饮及拆方对乳腺癌MDA-MB-231细胞增殖及凋亡的影响 |
| 投稿时间：2016-04-14 修订日期：2016-04-29 点此下载全文 |
| 引用本文：李凯,孙丽艳,许兴超,郭淑丽,李湘奇.乳康饮及拆方对乳腺癌MDA-MB-231细胞增殖及凋亡的影响[J].医学研究杂志,2016,45(12):32-35 |
| DOI：
10.11969/j.issn.1673-548X.2016.12.009 |
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| 基金项目:国家自然科学基金资助项目（81473687）；山东省自然科学基金资助项目（ZR2009CM039，ZR2013HM038）；山东省医药卫生科技发展计划项目（2011HW084） |
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| 中文摘要:目的 研究乳康饮及拆方对人三阴性乳腺癌细胞MDA-MB-231增殖抑制及诱导凋亡的作用。方法 采用乳康饮及拆方含药血清处理人三阴性乳腺癌细胞MDA-MB-231，分别在24、48、72h时间点，用cell counting kit-8（CCK-8法）检测乳康饮及拆方在不同时间点对MDA-MB-231细胞增殖的抑制作用，FCM法（流式细胞术）检测乳康饮及拆方诱导MDA-MB-231细胞凋亡。结果 各组中药处理48h后，细胞增殖出现明显抑制，抑制率分别为46.05%、31.85%、41.24%，相对氟尿嘧啶组抑制作用时间有明显延迟，乳康饮组在48h与氟尿嘧啶组抑制率之间差异无统计学意义（P=0.076）。各处理组48h凋亡率分别为40.7%、16.3%、23.2%、57.5%，比空白对照组凋亡率显著增加（P<0.05），乳康饮组与氟尿嘧啶组凋亡率差异无统计学意义，但高于拆方2组（P=0.000）。结论 乳康饮及其拆方能够显著抑制乳腺癌MDA-MB-231细胞增殖，并诱导其凋亡。 |
| 中文关键词:三阴性乳腺癌 乳康饮 细胞凋亡 细胞增殖 |
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| Effect of Rukangyin and Seperated Prescriptions on Proliferation and Apoptotic of MDA-MB-231 |
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| Abstract:Objective To study the effect of Rukangyin and its seperated prescriptions on the proliferation inhibition and apoptotic inducement of human triple negative breast cancer cells MDA-MB-231.Methods We treated human triple-negative breast cancer cells MDA-MB-231 at 24h, 48h, and 72h by using serum medicated in Rukangyin and its seperated prescriptions, and then tested the inhibiting effect of Rukangyin and its seperated prescriptions on the proliferation of MDA-MB-231 cells and induced these cells to be apoptotic at different times by using cell counting kit-8(CCK-8) and FCM respectively.Results After 48h of treatment with traditional Chinese medicine, significant inhibition of cell proliferation arose, whose inhibition ratio was 46.05%, 31.85% and 41.24%. Compared with Fluorouracil group, inhibition time significantly delayed, which declared that the difference of the inhibition ratio between Rukangyin group and Fluorouracil group at 48h was not statistically significant(P=0.076). The apoptosis ratio of each treatment group was 40.7%, 16.3%, 23.2% and 57.5% respectively, which showed a significant increase compared with the control groups(P<0.05), and it declared that apoptosis ratio between Rukangyin group and Fluorouracil group was not statistically significant, but they were higher than the two seperated prescription groups (P=0.000).Conclusion Rukangyin and its seperated prescriptions can significantly inhibit the proliferation and induce apoptosis of breast cancer MDA-MB-231 cells. |
| keywords:Triple negative breast cancer Rukangyin Cell apoptosis Cell proliferation |
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